Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Stefan A. Sonderhoff"'
Autor:
Stefan A. Sonderhoff, Phylis W. S. Pui, Martin Groeschl, Felix Trampler, Douglas G. Kilburn, James M. Piret
Publikováno v:
Biotechnology Progress. 11:146-152
Ultrasound was used to enhance the sedimentation of hybridoma cells from medium in a 75 mL resonator chamber. Forces in the acoustic standing waves aggregated the cells, and the aggregates were then rapidly sedimented by gravity. Cell separation incr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfd2f85ca6d32bf555d04389a85b5670
https://doi.org/10.1021/bp00032a005
https://doi.org/10.1021/bp00032a005
Publikováno v:
Biotechnology and Bioengineering. 39:859-864
Analysis of cellular ATP as a means of measuring viable biomass loading was investigated in hybridoma cell culture. ATP analysis by the luciferin-luciferase assay was compared with trypan blue-stained hemocytometer counts. The cell-specific ATP conte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c6e3d902bc633c63580c8b17d65d97e
https://doi.org/10.1002/bit.260390807
https://doi.org/10.1002/bit.260390807
Publikováno v:
Bio/technology (Nature Publishing Company). 12(3)
We have developed a flow-through device which uses high frequency, low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion bioreactor. The system retained up to 99 percent of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::837e82b8abe2bbcbd2c57187f3f0bbc1
https://pubmed.ncbi.nlm.nih.gov/7764489
https://pubmed.ncbi.nlm.nih.gov/7764489
Autor:
Phylis W. S. Pui, James M. Piret, Felix Trampler, Wolfgang Burger, Ewald Benes, Stefan A. Sonderhoff, Martin Groeschl, Douglas G. Kilburn
An acoustic resonator was used to generate an ultrasonic standing wave field within a suspension of hybridoma cells. Acoustically induced aggregation and increased cell sedimentation rates were observed. Exposure of cells to ultrasonic resonance fiel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e5ead994bc0b7fb700bf8702e87fbfb2
https://doi.org/10.1016/b978-0-7506-1877-9.50127-x
https://doi.org/10.1016/b978-0-7506-1877-9.50127-x
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