Common Considerations for Genotoxicity Assessment of Nanomaterials

Autor: Rosalie K. Elespuru, Shareen H. Doak, Andrew R. Collins, Maria Dusinska, Stefan Pfuhler, Mugimane Manjanatha, Renato Cardoso, Connie L. Chen

Publikováno v: Frontiers in Toxicology, Vol 4 (2022)

Genotoxicity testing is performed to determine potential hazard of a chemical or agent for direct or indirect DNA interaction. Testing may be a surrogate for assessment of heritable genetic risk or carcinogenic risk. Testing of nanomaterials (NM) for

Externí odkaz: https://doaj.org/article/2d21ba7877bd47e2a4f12d4d14fd3fcd

Measuring DNA modifications with the comet assay: a compendium of protocols

Autor: Andrew Collins, Peter Møller, Goran Gajski, Soňa Vodenková, Abdulhadi Abdulwahed, Diana Anderson, Ezgi Eyluel Bankoglu, Stefano Bonassi, Elisa Boutet-Robinet, Gunnar Brunborg, Christy Chao, Marcus S. Cooke, Carla Costa, Solange Costa, Alok Dhawan, Joaquin de Lapuente, Cristian Del Bo’, Julien Dubus, Maria Dusinska, Susan J. Duthie, Naouale El Yamani, Bevin Engelward, Isabel Gaivão, Lisa Giovannelli, Roger Godschalk, Sofia Guilherme, Kristine B. Gutzkow, Khaled Habas, Alba Hernández, Oscar Herrero, Marina Isidori, Awadhesh N. Jha, Siegfried Knasmüller, Ingeborg M. Kooter, Gudrun Koppen, Marcin Kruszewski, Carina Ladeira, Blanca Laffon, Marcelo Larramendy, Ludovic Le Hégarat, Angélique Lewies, Anna Lewinska, Guillermo E. Liwszyc, Adela López de Cerain, Mugimane Manjanatha, Ricard Marcos, Mirta Milić, Vanessa Moraes de Andrade, Massimo Moretti, Damian Muruzabal, Matjaž Novak, Rui Oliveira, Ann-Karin Olsen, Norah Owiti, Mário Pacheco, Alok K. Pandey, Stefan Pfuhler, Bertrand Pourrut, Kerstin Reisinger, Emilio Rojas, Elise Rundén-Pran, Julen Sanz-Serrano, Sergey Shaposhnikov, Ville Sipinen, Karen Smeets, Helga Stopper, João Paulo Teixeira, Vanessa Valdiglesias, Mahara Valverde, Frederique van Acker, Frederik-Jan van Schooten, Marie Vasquez, Johannes F. Wentzel, Maciej Wnuk, Annelies Wouters, Bojana Žegura, Tomas Zikmund, Sabine A. S. Langie, Amaya Azqueta

Publikováno v: Nature Protocols
Nature Protocols, 2023, ⟨10.1038/s41596-022-00754-y⟩
Nature Protocols. Nature Publishing Group
Nat Protoc

The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b143a2dbb2770d10bfa61ac51dd6ffd
https://doi.org/10.1038/s41596-022-00754-y

Use of the EpiDermTM 3D reconstructed skin micronucleus assay for fragrance materials

Autor: Marilyn J. Aardema, H. Moustakas, A.M. Api, Y. Thakkar, Shambhu K Roy, Stefan Pfuhler

Publikováno v: Mutagenesis. 37:89-111

In order to evaluate the utility of the 3D reconstructed skin micronucleus assay (3DRSMN) to assess clastogenic/aneugenic potential of the fragrance chemicals, a set of 22 fragrance materials were evaluated in 3DRSMN assay. These materials evaluated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a28d11669cb793f7b032a4b1acf5491
https://doi.org/10.1093/mutage/geab040

Quantitative in vitro to in vivo Extrapolation (IVIVE) of Genotoxicity Data Provides Protective Estimates of in vivo Dose

Autor: Marc A. Beal, Marc Audebert, Tara Barton‐Maclaren, Hannah Battaion, Jeffrey C. Bemis, Xuefei Cao, Connie Chen, Stephen D. Dertinger, Roland Froetschl, Xiaoqing Guo, George Johnson, Giel Hendriks, Laure Khoury, Alexandra S. Long, Stefan Pfuhler, Raja S. Settivari, Shamika Wickramasuriya, Paul White

Publikováno v: Environmental and molecular mutagenesis, Early View

Genotoxicity assessment is a critical component in the development and evaluation of chemicals. Traditional genotoxicity assays (i.e., mutagenicity, clastogenicity, aneugenicity) have been limited to dichotomous hazard classification, while other tox

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4a6d59ccfacfcd3ddb681a0a6174ca8
https://pubmed.ncbi.nlm.nih.gov/36495195

A weight of evidence review of the genotoxicity of titanium dioxide (TiO

Autor: David, Kirkland, Marilyn J, Aardema, Rüdiger V, Battersby, Carol, Beevers, Karin, Burnett, Arne, Burzlaff, Andreas, Czich, E Maria, Donner, Paul, Fowler, Helinor J, Johnston, Harald F, Krug, Stefan, Pfuhler, Leon F, Stankowski

Publikováno v: Regulatory toxicology and pharmacology : RTP. 136

Titanium dioxide is a ubiquitous white material found in a diverse range of products from foods to sunscreens, as a pigment and thickener, amongst other uses. Titanium dioxide has been considered no longer safe for use in foods (nano and microparticl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3514c69cd0fc7494178b71f4a43c7ae8
https://pubmed.ncbi.nlm.nih.gov/36228836