Zobrazeno 1 - 10
of 219
pro vyhledávání: '"Stefan, Kubick"'
Autor:
Jeffrey L. Schloßhauer, Lena Tholen, Alexander Körner, Stefan Kubick, Sofia Chatzopoulou, Anja Hönow, Anne Zemella
Publikováno v:
Synthetic and Systems Biotechnology, Vol 9, Iss 3, Pp 416-424 (2024)
Chinese hamster ovary (CHO) cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational modifications. However, toxic proteins and membrane proteins are often difficult-to-express in livin
Externí odkaz:
https://doaj.org/article/135f28cf17b64878a5997cacbe96703b
Autor:
Jan Felix Knauer, Christian Schulz, Anne Zemella, Doreen A. Wüstenhagen, Ruben Magnus Walter, Jan-Heiner Küpper, Stefan Kubick
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)
Abstract Cytochromes P450 (CYPs) are a group of monooxygenases that can be found in almost all kinds of organisms. For CYPs to receive electrons from co-substrate NADPH, the activity of NADPH-Cytochrome-P450-oxidoreductase (CPR) is required as well.
Externí odkaz:
https://doaj.org/article/54ec96165b0e49408cbc32198fc8fced
Autor:
Jeffrey L. Schloßhauer, Anne Zemella, Srujan K. Dondapati, Lena Thoring, Manpreet Meyer, Stefan Kubick
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract Modification of proteins with a broad range of chemical functionalities enables the investigation of protein structure and activity by manipulating polypeptides at single amino acid resolution. Indeed, various functional groups including bul
Externí odkaz:
https://doaj.org/article/9837d159f19f4fed8152db056c167e33
Autor:
Paria Pouyan, Anne Zemella, Jeffrey L. Schloßhauer, Ruben M. Walter, Rainer Haag, Stefan Kubick
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract With more than 20 Food and Drug Administration (FDA)-approved poly (ethylene glycol) (PEG) modified drugs on the market, PEG is the gold standard polymer in bioconjugation. The coupling improves stability, efficiency and can prolong blood ci
Externí odkaz:
https://doaj.org/article/f1014fa041b2467d9150463401d73426
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)
Abstract Understanding the assembly mechanism and function of membrane proteins is a fundamental problem in biochemical research. Among the membrane proteins, G protein-coupled receptors (GPCRs) represent the largest class in the human body and have
Externí odkaz:
https://doaj.org/article/8dbffe84205e4e0ebbcfe2d4e7e28e7a
Publikováno v:
Bioengineering, Vol 11, Iss 1, p 92 (2024)
Cell-free systems are particularly attractive for screening applications and the production of difficult-to-express proteins. However, the production of cell lysates is difficult to implement on a larger scale due to large time requirements, cultivat
Externí odkaz:
https://doaj.org/article/7170885bf2d04ba6a158132985c7d758
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Unspecific peroxygenases (UPOs, EC 1.11.2.1) are fungal enzymes that catalyze the oxyfunctionalization of non-activated hydrocarbons, making them valuable biocatalysts. Despite the increasing interest in UPOs that has led to the identification of tho
Externí odkaz:
https://doaj.org/article/85ab186ff1164aa09f46647d7189e683
Publikováno v:
Cells, Vol 12, Iss 17, p 2140 (2023)
In the liver, phase-1 biotransformation of drugs and other xenobiotics is largely facilitated by enzyme complexes consisting of cytochrome P450 oxidoreductase (CPR) and cytochrome P450 monooxygenases (CYPs). Generated from human liver-derived cell li
Externí odkaz:
https://doaj.org/article/bc79494e051f4242b354ca3984c39aa4
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 7, p 6299 (2023)
Oligomeric ion channels are abundant in nature. However, the recombinant expression in cell culture-based systems remains tedious and challenging due to negative side effects, limiting the understanding of their role in health and disease. Accordingl
Externí odkaz:
https://doaj.org/article/f9e6368a7b2741fca7f1efb52143744b
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Cell-free systems are well-established platforms for the rapid synthesis, screening, engineering and modification of all kinds of recombinant proteins ranging from membrane proteins to soluble proteins, enzymes and even toxins. Also within the antibo
Externí odkaz:
https://doaj.org/article/edebfd26b0cc452ba64ebc49bda404b9