Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity.

Autor: Hoving D; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. d.hoving@lumc.nl., Marques AHC; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Huisman W; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Nosoh BA; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., de Kroon AC; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., van Hengel ORJ; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Wu BR; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Steenbergen RAM; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., van Helden PM; AIMM Therapeutics, Amsterdam, The Netherlands., Urban BC; Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, UK.; Oxford Vaccine Group, University of Oxford, Oxford, UK., Dhar N; Vaccines & Infectious Diseases Analytics, University of Witwatersrand, Johannesburg, South Africa., Ferreira DM; Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, UK.; Oxford Vaccine Group, University of Oxford, Oxford, UK., Kwatra G; Vaccines & Infectious Diseases Analytics, University of Witwatersrand, Johannesburg, South Africa.; Department of Clinical Microbiology, Christian Medical College, Vellore, India., Hokke CH; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Jochems SP; Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. s.p.jochems@lumc.nl.

Publikováno v: Communications biology [Commun Biol] 2023 Oct 28; Vol. 6 (1), pp. 1095. Date of Electronic Publication: 2023 Oct 28.