Zobrazeno 1 - 10
of 364
pro vyhledávání: '"Stanley T Crooke"'
Publikováno v:
PLoS ONE, Vol 12, Iss 3, p e0173494 (2017)
Altered expression of NEAT1, the architectural long non-coding RNA (lncRNA) of nuclear paraspeckles, has been reported during tumorigenesis, as well as under various cellular stress conditions. Here we report that the depletion of NEAT1 lncRNA allevi
Externí odkaz:
https://doaj.org/article/2e846a35e03b475cbf12c990bc56d2b4
Autor:
Timothy A Vickers, Stanley T Crooke
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0161930 (2016)
Protein-nucleic acid interactions play a crucial role in the regulation of diverse biological processes. Elucidating the roles that protein-nucleic acid complexes play in the regulation of transcription, translation, DNA replication, repair and recom
Externí odkaz:
https://doaj.org/article/f3b1bc87cb654ba7b7b5cb800ffba823
Publikováno v:
PLoS ONE, Vol 9, Iss 10, p e110615 (2014)
A new strategy for identifying potent RNase H-dependent antisense oligonucleotides (ASOs) is presented. Our analysis of the human transcriptome revealed that a significant proportion of genes contain unique repeated sequences of 16 or more nucleotide
Externí odkaz:
https://doaj.org/article/96ce270b7c0e48a58c215806e264aeed
Autor:
Timothy A Vickers, Stanley T Crooke
Publikováno v:
PLoS ONE, Vol 9, Iss 10, p e108625 (2014)
Antisense oligonucleotides (ASOs) are most commonly designed to reduce targeted RNA via RNase H1-dependent degradation. In this paper we demonstrate that cellular proteins can compete for sites targeted by RNase H1-dependent ASOs. We further show tha
Externí odkaz:
https://doaj.org/article/3e2c841fcdf541a085ac643d5b7f6fe1
Publikováno v:
PLoS ONE, Vol 9, Iss 7, p e101752 (2014)
To better understand the factors that influence the activity and specificity of antisense oligonucleotides (ASOs), we designed a minigene encoding superoxide dismutase 1 (SOD-1) and cloned the minigene into vectors for T7 transcription of pre-mRNA an
Externí odkaz:
https://doaj.org/article/2c773100b2994ebfb9f9401e512d914e
Publikováno v:
PLoS ONE, Vol 8, Iss 8, p e71006 (2013)
Mammalian RNase H1 has been implicated in mitochondrial DNA replication and RNA processing and is required for embryonic development. We identified the mitochondrial protein P32 that binds specifically to human RNase H1, but not human RNase H2. P32 b
Externí odkaz:
https://doaj.org/article/da23a5af270d45d1a9b23c134f067f1f
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 33, Iss , Pp 832-844 (2023)
Single-stranded phosphorothioate oligonucleotides (PS-oligos) can activate TLR9, leading to an innate immune response. This can occur with PS-oligos containing unmethylated CpG sites, the canonical motif, or PS-oligos that do not contain those motifs
Externí odkaz:
https://doaj.org/article/4f3cba99e8d34c71ad01fbcae30d0bc0
Publikováno v:
Nucleic Acid Therapeutics. 33:95-107
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::049cdb549c72a0b364f5a1a7e226fdad
https://doi.org/10.1089/nat.2022.0067
https://doi.org/10.1089/nat.2022.0067
Autor:
Lingdi Zhang, Xue-hai Liang, Cheryl Li De Hoyos, Michael Migawa, Joshua G. Nichols, Graeme Freestone, Jun Tian, Punit P. Seth, Stanley T. Crooke
Publikováno v:
Nucleic Acid Therapeutics. 32:401-411
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d010ee80d944483c78c624b02605c89a
https://doi.org/10.1089/nat.2022.0005
https://doi.org/10.1089/nat.2022.0005
Autor:
Lingdi, Zhang, Karla D, Bernardo, Timothy A, Vickers, Jun, Tian, Xue-Hai, Liang, Stanley T, Crooke
Publikováno v:
Nucleic Acid Therapeutics. 32:280-299
RNase H1-dependent phosphorothioate oligonucleotides (PS-ASOs) have been developed to treat various diseases through specific degradation of target RNAs. Although many factors or features of RNA and PS-ASOs have been demonstrated to affect antisense
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb03ace940b5cc82808a2687b1c11502
https://doi.org/10.1089/nat.2021.0107
https://doi.org/10.1089/nat.2021.0107