Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Stanley R. Maloy"'
Autor:
Robert A. Edwards, Stanley R. Maloy
Publikováno v:
BioTechniques, Vol 30, Iss 2, Pp 304-311 (2001)
Salmonella are intracellular pathogens that infect and multiply inside macrophages. Although Salmonella are some of the beststudied pathogens, it is difficult to determine quickly and reliably whether the bacteria are intracellular or extracellular.
Externí odkaz:
https://doaj.org/article/e55fa89ca76846438167f0e7797148fd
Publikováno v:
BioTechniques, Vol 26, Iss 5, Pp 892-900 (1999)
E. coli and Salmonella typhimurium are widely used bacterial hosts for genetic manipulation of DNA from prokaryotes and eukaryotes. Introduction of foreign DNA by electroporation or transduction into E. coli and Salmonella is limited by host restrict
Externí odkaz:
https://doaj.org/article/d1e15161986041158691d2e0268a19f3
Publikováno v:
International Journal of Microbiology, Vol 2010 (2010)
Many bacteria produce secreted virulence factors called exotoxins. Exotoxins are often encoded by mobile genetic elements, including bacteriophage (phage). Phage can transfer genetic information to the bacteria they infect. When a phage transfers vir
Externí odkaz:
https://doaj.org/article/eb13df62edf3419c945afaf30b825f7b
Autor:
Stanley R, Maloy
Publikováno v:
Methods in enzymology. 421
Transposon insertions in and near a gene of interest facilitate the genetic characterization of a gene in vivo. This chapter is dedicated to describing the isolation of mini-Tn10 insertions in any desired nonessential gene in Salmonella enterica, as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::54fab31abc92d711894d89d3147a81ba
https://pubmed.ncbi.nlm.nih.gov/17352913
https://pubmed.ncbi.nlm.nih.gov/17352913
Autor:
Stanley R, Maloy
Publikováno v:
Methods in enzymology. 421
One of the greatest advances in molecular genetics has been the application of selectable transposons in molecular biology. After 30 years of use in microbial genetics studies, transposons remain indispensable tools for the generation of null alleles
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c9d917138a9cee959234d2d872cf4fb0
https://pubmed.ncbi.nlm.nih.gov/17352910
https://pubmed.ncbi.nlm.nih.gov/17352910
Autor:
Stanley R, Maloy
Publikováno v:
Methods in enzymology. 421
Localized mutagenesis can be used to obtain mutants in genes of interest based on linkage to selectable markers. Mutagens diethylsulfate and hydroxylamine are used to obtain predominantly transition mutations in the DNA either by whole chromosomal mu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e702c654601b8799c4aab119e5d7bfdf
https://pubmed.ncbi.nlm.nih.gov/17352914
https://pubmed.ncbi.nlm.nih.gov/17352914
Autor:
Stanley R, Maloy, Kelly T, Hughes
Publikováno v:
Methods in enzymology. 421
The ease of rapidly accumulating a large number of mutants requires careful bookkeeping to avoid confusing one mutant with another. Each mutant constructed should be assigned a strain number. Strain numbers usually consist of two to three capital let
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6a96b5bda6c0d22054ac2582f516a71a
https://pubmed.ncbi.nlm.nih.gov/17352909
https://pubmed.ncbi.nlm.nih.gov/17352909
Autor:
Stanley R, Maloy, Jeffrey, Gardner
Publikováno v:
Methods in enzymology. 421
The bacteriophage P22-based challenge system is a sophisticated genetic tool for the characterization of sequence-specific recognition of DNA and RNA in vivo. The construction of challenge phage follows simple phage lysate preparations and detection
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c7cd0adcb4823dbecd1c63985cc700a0
https://pubmed.ncbi.nlm.nih.gov/17352926
https://pubmed.ncbi.nlm.nih.gov/17352926
Autor:
Jenny A, Crain, Stanley R, Maloy
Publikováno v:
Methods in enzymology. 421
Mud-P22 derivatives are hybrids between phage Mu and P22 that can be inserted at essentially any desired site on the Salmonella chromosome (Benson and Goldman, 1992; Youderian et al., 1988). Induction of Mud-P22 insertions yields phage particles that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cc93cda28e448e648f7f8488ac0ceb9c
https://pubmed.ncbi.nlm.nih.gov/17352927
https://pubmed.ncbi.nlm.nih.gov/17352927
