Zobrazeno 1 - 10
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pro vyhledávání: '"Staffan Bergh"'
Autor:
Stewart T. Cole, Staffan Bergh
Publikováno v:
Molecular Microbiology. 12:517-534
As part of ongoing efforts to investigate the molecular biology of the human pathogens in the genus Mycobacterium, a customized database was developed specifically for these organisms and implemented in ACEDB database manager software. The data loade
Publikováno v:
Electrophoresis. 13:547-551
An integrated protocol for solid-phase DNA sequencing using a robotic work station is described involving magnetic separation of DNA and analysis of the sequencing product by electrophoresis with automated detection of the fluorescently labeled fragm
Autor:
Staffan Bergh, Johan Wahlberg, Mathias Uhlén, Joakim Lundeberg, Bertil Petterson, Anders Holmberg, Tomas Moks, Thomas Hultman, Stefan Ståhl
Publikováno v:
Trends in Biotechnology. 10:52-55
Increasing the efficiency of DNA sequencing necessitates the development of systems which reduce the need for manual operations by integrating template preparation, sequencing reactions, product separation and detection. A semi-automated system, wher
Publikováno v:
Bacterial Genomes ISBN: 9781461379256
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::092b1b8ea4cac4e2a523dc3642584137
https://doi.org/10.1007/978-1-4615-6369-3_68
https://doi.org/10.1007/978-1-4615-6369-3_68
Autor:
Staffan Bergh, Stewart T. Cole
Publikováno v:
Bacterial Genomes ISBN: 9781461379256
In recent years there has been a considerable upturn in interest in the genetics and microbiology of the mycobacteria (Young and Cole, 1993), and the reasons for this are two-fold. Firstly, after several decades of relative tranquility, mycobacterial
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4e0cfac06c8baabb6edd4ac17435a2ed
https://doi.org/10.1007/978-1-4615-6369-3_39
https://doi.org/10.1007/978-1-4615-6369-3_39
Autor:
Temduang Limpaiboon, Nadine Honoré, Staffan Bergh, K. Eiglmeir, P. R. Ridel, S. Newton, G. R. Ramesh, S. Chanteau, N. Sittisombut, F. Doucet‐Populaire, P. del Portillo, Stewart T. Cole, P. Launois, S. Wu‐Hunte, C. Georges, Thierry Garnier, K. Niang, Prabhakara P. Reddi
Publikováno v:
Molecular microbiology. 7(2)
The nucleotide sequence of cosmid B1790, carrying the Rif-Str regions of the Mycobacterium leprae chromosome, has been determined. Twelve open reading frames were identified in the 36716bp sequence, representing 40% of the coding capacity. Five ribos
Autor:
Mathias Uhlén, Staffan Bergh
Publikováno v:
Gene. 117(1)
A gene cluster homologous to the β-ketoacyl synthase-encoding gene, actl, was cloned by Southern blot hybridization from Streptomyces curacoi, and a 5469-bp fragment was sequenced. Analysis of the sequence revealed seven open reading frames (ORFs).
Autor:
Staffan Bergh, Mathias Uhlén
Publikováno v:
Biotechnology and applied biochemistry. 15(1)
Streptomyces curacoi produces curamycin, an antibiotic based on a modified orsellinic acid skeleton that is synthesized by the polyketide pathway. We have cloned, characterized, and partly sequenced a polyketide synthase gene cluster of S. curacoi. T
Colorimetric quantification of in vitro-amplified template DNA to be used for solid phase sequencing
Publikováno v:
DNA sequence : the journal of DNA sequencing and mapping. 2(2)
A protocol for colorimetric determination of DNA amplified by the polymerase chain reaction (PCR) and subsequently immobilized to a solid support is described. The protocol consists of three steps: (i) binding of PCR amplified lac operator-containing
Autor:
Kathleen Falls, Laura Richterich, Steven McDougall, Craig Deloughery, Ronald Lundstrom, Deepika Madan, Craig Tulig, Joan Imrich, Dana Torrey, Gary L. Breton, Philip W. Rice, Stewart T. Cole, Keith Robison, Marcy Engelstein, Marc J. Rubenfield, Carol Butler, Karin Eiglmeier, Peter Richterich, Raymond M. Nietupski, Lynn Doucette-Stamm, Kari Abendschan, Maria Chung, Susan Kirst, Rene Gibson, Hershel Safer, Steven Connelly, George M. Church, Staffan Bergh, Tyler J. Aldredge, Qinxue Xu, Bruce Seitz, Hongmei Lee, Jen-i Mao, Jason M. Johnson, Kristin Gundersen, James W. Maher, Douglas R. Smith
Publikováno v:
Scopus-Elsevier
The nucleotide sequence of 1.5 Mb of genomic DNA fromMycobacterium leprae was determined using computer-assisted multiplex sequencing technology. This brings the 2.8-Mb M. leprae genome sequence to ∼66% completion. The sequences, derived from 43 re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8b6d2e156de9272a9fa93f741d360ad
https://infoscience.epfl.ch/record/151290
https://infoscience.epfl.ch/record/151290