Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Stacy A. Overman"'
Autor:
Edward H. Egelman, George J. Thomas, Masamichi Tsuboi, Ying A. Wang, Xiong Yu, Stacy A. Overman
Publikováno v:
Journal of Molecular Biology. 361:209-215
Many thin helical polymers, including bacterial pili and filamentous bacteriophage, have been seen as refractory to high-resolution studies by electron microscopy. Studies of the quaternary structure of such filaments have depended upon techniques su
Publikováno v:
Biochemistry. 44:3091-3100
The filamentous bacteriophage PH75, which infects the thermophile T. thermophilus, assembles in vivo at 70 degrees C and is stable to at least 90 degrees C. Although a high-resolution structure of PH75 is not available, the virion is known to compris
Publikováno v:
Journal of Molecular Structure. :271-278
3-Methylindole (3MI), which serves as a structural model for the tryptophan side chain in proteins, has been investigated using vapor phase Raman spectroscopy. The vapor phase spectrum of 3MI identifies the Raman signature of the indolyl moiety free
Publikováno v:
Journal of Raman Spectroscopy. 36:279-299
Applications of Raman spectroscopy to investigate the molecular constituents of nucleic acids were initiated in the late 1960s and soon thereafter progressed to studies of synthetic and native nucleic acids and complex biological assemblies containin
Autor:
George J. Thomas, Arantxa Rodriguez-Casado, Masamichi Tsuboi, Koji Nakamura, Stacy A. Overman
Publikováno v:
Biophysical Journal. 84:1969-1976
The filamentous bacteriophage Pf3 consists of a covalently closed DNA single strand of 5833 nucleotides sheathed by approximately 2500 copies of a 44-residue capsid subunit. The capsid subunit contains a single tryptophan residue (Trp-38), which is l
Publikováno v:
Biochemistry. 40:2522-2529
p-Cresol is a simple molecular model for the para phenolic side chain of tyrosine. Previously, Siamwiza and co-workers [(1975) Biochemistry 14, 4870-4876] investigated p-cresol solutions to identify Raman spectroscopic signatures for different hydrog
Publikováno v:
Biochemistry. 40:449-458
The Pseudomonas bacteriophage Pf3 is a long and narrow filament consisting of a covalently closed DNA single strand of 5833 bases sheathed by approximately 2500 copies of a 44-residue subunit. Ultraviolet resonance Raman spectra excited at 257, 244,
Publikováno v:
Biophysical Journal. 74(6):3217-3225
Virions of the Ff group of bacteriophages (fd, f1, M13) are morphologically identical filaments (approximately 6-nm diameter x approximately 880-nm length) in which a covalently closed, single-stranded DNA genome is sheathed by approximately 2700 cop
Autor:
George J. Thomas, Stacy A. Overman
Publikováno v:
Biochemistry. 37:5654-5665
The filamentous virus fd consists of a single-stranded DNA genome sheathed by 2700 copies of a 50-residue alpha-helical subunit (protein pVIII) and serves as a model assembly of alpha-helices. To advance vibrational assignments for the alpha-helix, w
Autor:
Olivia Osman, Kelly L. Aubrey, George J. Thomas, Kim E. Reilly, Shirley J. Hayes, Stacy A. Overman, Philip Serwer
Publikováno v:
Biospectroscopy. 4:S47-S56
The structure of the packaged double-stranded DNA genome of bacteriophage T7 was compared to that of unpackaged T7 DNA using digital difference Raman spectroscopy. Spectral data were obtained at 25 degrees C from native T7 virus (100 mg/mL), empty T7