A panel of blood-derived miRNAs with a stable expression pattern as a potential pan-cancer detection signature

Autor: Amir Sabbaghian, Veronika Mussack, Benedikt Kirchner, Maria L. U. Bui, Mohammad Reza Kalani, Michael W. Pfaffl, Masoud Golalipour

Publikováno v: Frontiers in Molecular Biosciences, Vol 9 (2022)

Introduction: MicroRNAs have a significant role in the regulation of the transcriptome. Several miRNAs have been proposed as potential biomarkers in different malignancies. However, contradictory results have been reported on the capability of miRNA

Externí odkaz: https://doaj.org/article/8d680e04f82b48da8fc207f194ae9965

Validation of Reference Genes for Quantifying Changes in Physiological Gene Expression in Apple Tree under Cold Stress and Virus Infection

Autor: Ju-Yeon Yoon, Jae-Hoon Jeong, Seung-Kook Choi

Publikováno v: Research in Plant Disease, Vol 26, Iss 3, Pp 144-158 (2020)

Quantitative reverse transcription PCR is used for gene expression analysis as the accurate and sensitive method. To analyze quantification of gene expression changes in apple plants, 10 housekeeping genes (ACT, CKL, EF-1α, GAPDH, MDH, PDI, THFs, UB

Externí odkaz: https://doaj.org/article/275af6b9b6f64f34842b5d5db53fe5b5

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Characterisation and validation of housekeeping genes for qRT-PCR expression analysis in Pterophyllum scalare

Autor: Ngairangbam Sushila, K. Pani Prasad, Basanta Kumar Das, Gayatri Tripathi

Publikováno v: Aquaculture International. 29:2387-2402

Quantitative real-time RT-PCR (qRT-PCR) is the most sensitive and advanced method to quantify the expression of target genes of animals including fish. However, the broad variation in the expression patterns of housekeeping genes (HKGs) among tissues

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cacde0f861991c382176bcf4ecceccf5
https://doi.org/10.1007/s10499-021-00754-x

DNA-induced spatial entrapment of general transcription machinery can stabilize gene expression in a nondividing cell

Autor: Khayam Javed, Jerome Jullien, Gaurav Agarwal, Nicola Lawrence, Richard Butler, Pantelis Savvas Ioannou, Farhat Nazir, J. B. Gurdon

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (4), pp.e2116091119. ⟨10.1073/pnas.2116091119⟩

Significance How differentiated cells such as muscle or nerve maintain their gene expression for prolonged times is currently elusive. Here, using Xenopus oocyte, we have shown that the stability of gene expression in nondividing cells may arise due

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::237b1d12f39c3f37f51eb0351e5cbbc7