Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Srivatsan Raman"'
Publikováno v:
eLife, Vol 12 (2024)
New experimental findings continue to challenge our understanding of protein allostery. Recent deep mutational scanning study showed that allosteric hotspots in the tetracycline repressor (TetR) and its homologous transcriptional factors are broadly
Externí odkaz:
https://doaj.org/article/e9712e3793624c2282368ace85ea09af
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Epistasis plays an important role in the evolution of novel protein functions because it determines the mutational path a protein takes. Here, the authors combine functional, structural and biophysical analyses to characterize epistasis in a computat
Externí odkaz:
https://doaj.org/article/40550d62365e4883ad8995105966c5fc
Publikováno v:
eLife, Vol 11 (2022)
A fundamental question in protein science is where allosteric hotspots – residues critical for allosteric signaling – are located, and what properties differentiate them. We carried out deep mutational scanning (DMS) of four homologous bacterial
Externí odkaz:
https://doaj.org/article/bb5c1278379443f2a4bf5d1f04daec34
Publikováno v:
eLife, Vol 10 (2021)
The interaction between a bacteriophage and its host is mediated by the phage's receptor binding protein (RBP). Despite its fundamental role in governing phage activity and host range, molecular rules of RBP function remain a mystery. Here, we system
Externí odkaz:
https://doaj.org/article/5883dcf2873c423a97d212c511324197
This upload conatins the raw fastq files for the manuscript "Rugged fitness landscapes minimize promiscuity in the evolution of transcriptional repressors". Deep sequencing was used to evaluate presorted and repressed sorted LacI-GalR Family (LGF) an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37f8e9d3e0c84e96c544c11300f3c2fe
Autor:
Phil Huss, Kristopher Kieft, Anthony Meger, Kyle Nishikawa, Karthik Anantharaman, Srivatsan Raman
Publikováno v:
bioRxiv
Bacteriophages can adapt to new hosts by altering sequence motifs through recombination or convergent evolution. Where such motifs exist and what fitness advantage they confer remains largely unknown. We report a new method, Bacteriophage Library Inf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7856dd6d59fd199e087108aa50b32f1
https://doi.org/10.1101/2023.02.07.527309
https://doi.org/10.1101/2023.02.07.527309
The ability to regulate circuits and pathways is central to cellular control. The existing toolkit is predominantly comprised of local transcription regulators that are unsuitable for exerting control at a global genome-wide scale. Bacterial sigma fa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2b8556206112517731a5935f6e183e9f
https://doi.org/10.1101/2022.11.29.518332
https://doi.org/10.1101/2022.11.29.518332
Publikováno v:
Trends in biochemical sciences.
Bacteriophage research has been vital to fundamental aspects of modern biology. Advances in metagenomics have revealed treasure troves of new and uncharacterized bacteriophages ('phages') that are not yet understood. However, our ability to find new
Publikováno v:
eLife. 11
A fundamental question in protein science is where allosteric hotspots – residues critical for allosteric signaling – are located, and what properties differentiate them. We carried out deep mutational scanning (DMS) of four homologous bacterial