Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Sriram Ramakrishnan"'
Publikováno v:
Applied Biochemistry and Biotechnology. :499-508
Chlorobium thiosulfatophilum may be used for the bioconversion of hydrogen sulfide to elemental sulfur or sulfate. Sulfur is the preferred product because of problems in the disposal of sulfate. A CSTR with a sulfur-settling separator has been used t
Publikováno v:
Molecular Therapy. 11:S221-S222
Top of pageAbstract Introduction: As the number of gene therapy studies and trials has increased in the past few years, an ever-increasing demand for plasmid DNA-based products exists. In this expanding field of plasmid production, emphasis has alway
Publikováno v:
Molecular Therapy. 9:S310-S311
Cost-effective production of commercial-scale batches of pharmaceutical-grade plasmid DNA remains a challenge for the maturing industry of plasmid-based biologics. A process is required that meets the quality standards for human pharmaceutical produc
Autor:
Justin Miller, Jeffrey Darnell, Gary Gates, Sriram Ramakrishnan, Hugo L. Gonzales, Henry Hebel
Publikováno v:
Molecular Therapy. 13:S121
The demand for high purity plasmid DNA for use in the fields of gene therapy and DNA vaccination is escalating rapidly. The new and expanding field of RNAi also relies on plasmids to express short double-stranded RNA molecules that specifically targe
Publikováno v:
Molecular Therapy. 11:S71
Adequate work has been performed on fermentation process optimizations for the production of plasmid DNA. However, the effects of dissolved oxygen (DO) control parameters in fermenters have not been addressed. DO control in high cell density fermenta
Publikováno v:
Molecular Therapy. 11:S70
Low-shear, alkaline-lysis procedures for cell lysis have been well-described in the literature. These methods avoid mechanical damage to the plasmid and other impurities. Further, low-shear methods for lysate neutralization and subsequent removal of
Publikováno v:
Molecular Therapy. 11:S71
There is high demand for high purity plasmid DNA for use in the fields of gene therapy and DNA vaccination. A need also exists for a cost-effective process that significantly reduces the time and expense of current plasmid production technologies. Ou
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