Multiple serine transposase dimers assemble the transposon-end synaptic complex during IS607-family transposition

Autor: Wenyang Chen, Sridhar Mandali, Stephen P Hancock, Pramod Kumar, Michael Collazo, Duilio Cascio, Reid C Johnson

Publikováno v: eLife, Vol 7 (2018)

IS607-family transposons are unusual because they do not have terminal inverted repeats or generate target site duplications. They encode two protein-coding genes, but only tnpA is required for transposition. Our X-ray structures confirm that TnpA is

Externí odkaz: https://doaj.org/article/ffeffce569774af6adcaf2932fc06da3

The HMGB chromatin protein Nhp6A can bypass obstacles when traveling on DNA

Autor: Satoshi Takahashi, Kana Ouchi, Yining Wu, Eriko Mano, Reid C. Johnson, Kiyoto Kamagata, Cheng Tan, Shoji Takada, Sridhar Mandali

Publikováno v: Nucleic Acids Research

DNA binding proteins rapidly locate their specific DNA targets through a combination of 3D and 1D diffusion mechanisms, with the 1D search involving bidirectional sliding along DNA. However, even in nucleosome-free regions, chromosomes are highly dec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa9f4029419dab754f0f93473fcfe5bc
https://doi.org/10.1093/nar/gkaa799

Ubiquitous Selfish Toxin-Antidote Elements in Caenorhabditis Species

Autor: Philipp Verpukhovskiy, Leonid Kruglyak, Tzitziki Lemus-Vergara, Eyal Ben-David, Sridhar Mandali, Pinelopi Pliota, Alejandro Burga, Christian Braendle, Alevtina Koreshova, Sonya A. Widen

Publikováno v: Current Biology

Summary Toxin-antidote elements (TAs) are selfish genetic dyads that spread in populations by selectively killing non-carriers. TAs are common in prokaryotes, but very few examples are known in animals. Here, we report the discovery of maternal-effec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::724da882f52b594900a2df4a97873635

Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase

Autor: Kushol Gupta, Anthony R. Dawson, Reid C. Johnson, Gregory D. Van Duyne, Sridhar Mandali

Publikováno v: Journal of bacteriology, vol 199, iss 11

The serine integrase of phage A118 catalyzes integrative recombination between attP on the phage and a specific attB locus on the chromosome of Listeria monocytogenes , but it is unable to promote excisive recombination between the hybrid attL and at

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2ca6ae6bd008427c466bd302d3d7775
https://escholarship.org/uc/item/1423t03w

The site-specific integration reaction of Listeria phage A118 integrase, a serine recombinase

Autor: Sridhar Mandali, Michael J. Haykinson, Reid C. Johnson, Gautam Dhar, Nuraly K. Avliyakulov

Publikováno v: Mandali, Sridhar; Dhar, Gautam; Avliyakulov, Nuraly K; Haykinson, Michael J; & Johnson, Reid C. (2013). The site-specific integration reaction of Listeria phage A118 integrase, a serine recombinase. Mobile DNA, 4(1), 2. doi: http://dx.doi.org/10.1186/1759-8753-4-2. Retrieved from: http://www.escholarship.org/uc/item/49c711v2
Mobile DNA
Mobile DNA, Vol 4, Iss 1, p 2 (2013)

Background A large subfamily of serine recombinases contains long polypeptide segments appended to the C-terminal end of the conserved catalytic domain. Members of this subfamily often function as phage integrases but also mediate transposition and r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1afbf906a00489212b78df4203cd4be
http://www.escholarship.org/uc/item/49c711v2