Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.

Autor: Altincekic N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Korn SM; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Qureshi NS; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Dujardin M; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Ninot-Pedrosa M; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Abele R; Institute for Biochemistry, Goethe University Frankfurt, Frankfurt am Main, Germany., Abi Saad MJ; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Alfano C; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Almeida FCL; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Alshamleh I; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., de Amorim GC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Multidisciplinary Center for Research in Biology (NUMPEX), Campus Duque de Caxias Federal University of Rio de Janeiro, Duque de Caxias, Brazil., Anderson TK; Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI, United States., Anobom CD; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Anorma C; Department of Chemistry, University of California, Irvine, CA, United States., Bains JK; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Bax A; LCP, NIDDK, NIH, Bethesda, MD, United States., Blackledge M; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Blechar J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Böckmann A; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Brigandat L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Bula A; Latvian Institute of Organic Synthesis, Riga, Latvia., Bütikofer M; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Camacho-Zarco AR; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Carlomagno T; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany.; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Braunschweig, Germany., Caruso IP; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Multiuser Center for Biomolecular Innovation (CMIB), Department of Physics, São Paulo State University (UNESP), São José do Rio Preto, Brazil., Ceylan B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Chaikuad A; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Chu F; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Cole L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Crosby MG; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., de Jesus V; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Dhamotharan K; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Felli IC; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Ferner J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Fleischmann Y; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Fogeron ML; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Fourkiotis NK; Department of Pharmacy, University of Patras, Patras, Greece., Fuks C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Fürtig B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Gallo A; Department of Pharmacy, University of Patras, Patras, Greece., Gande SL; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Gerez JA; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Ghosh D; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Gomes-Neto F; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Laboratory of Toxinology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil., Gorbatyuk O; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Guseva S; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Hacker C; Signals GmbH & Co. KG, Frankfurt am Main, Germany., Häfner S; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Hao B; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Hargittay B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Henzler-Wildman K; Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI, United States., Hoch JC; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Hohmann KF; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Hutchison MT; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Jaudzems K; Latvian Institute of Organic Synthesis, Riga, Latvia., Jović K; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Kaderli J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Kalniņš G; Latvian Biomedical Research and Study Centre, Riga, Latvia., Kaņepe I; Latvian Institute of Organic Synthesis, Riga, Latvia., Kirchdoerfer RN; Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI, United States., Kirkpatrick J; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany.; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Braunschweig, Germany., Knapp S; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Krishnathas R; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Kutz F; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Zur Lage S; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Braunschweig, Germany., Lambertz R; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Lang A; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Laurents D; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Lecoq L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Linhard V; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Löhr F; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute of Biophysical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany., Malki A; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Bessa LM; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Martin RW; Department of Chemistry, University of California, Irvine, CA, United States.; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., Matzel T; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Maurin D; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., McNutt SW; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Mebus-Antunes NC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Meier BH; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Meiser N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Mompeán M; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Monaca E; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Montserret R; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Mariño Perez L; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Moser C; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Muhle-Goll C; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Neves-Martins TC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Ni X; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Norton-Baker B; Department of Chemistry, University of California, Irvine, CA, United States., Pierattelli R; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Pontoriero L; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Pustovalova Y; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Ohlenschläger O; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Orts J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Da Poian AT; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Pyper DJ; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Richter C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Riek R; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Rienstra CM; Department of Biochemistry and National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, United States., Robertson A; LCP, NIDDK, NIH, Bethesda, MD, United States., Pinheiro AS; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Sabbatella R; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Salvi N; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Saxena K; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Schulte L; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Schiavina M; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Schwalbe H; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Silber M; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Almeida MDS; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Sprague-Piercy MA; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., Spyroulias GA; Department of Pharmacy, University of Patras, Patras, Greece., Sreeramulu S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Tants JN; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Tārs K; Latvian Biomedical Research and Study Centre, Riga, Latvia., Torres F; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Töws S; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Treviño MÁ; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Trucks S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Tsika AC; Department of Pharmacy, University of Patras, Patras, Greece., Varga K; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Wang Y; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany., Weber ME; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Weigand JE; Department of Biology, Technical University of Darmstadt, Darmstadt, Germany., Wiedemann C; Institute of Biochemistry and Biotechnology, Charles Tanford Protein Centre, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany., Wirmer-Bartoschek J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Wirtz Martin MA; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Zehnder J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Hengesbach M; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Schlundt A; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany.

Publikováno v: Frontiers in molecular biosciences [Front Mol Biosci] 2021 May 10; Vol. 8, pp. 653148. Date of Electronic Publication: 2021 May 10 (Print Publication: 2021).

Chemical Properties Determine Solubility and Stability in βγ-Crystallins of the Eye Lens.

Autor: Rocha MA; Department of Chemistry, University of California, Irvine, 1102 Natural Sciences 2, Irvine, CA, 92697-2025, USA., Sprague-Piercy MA; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697-3900, USA., Kwok AO; Department of Chemistry, University of California, Irvine, 1102 Natural Sciences 2, Irvine, CA, 92697-2025, USA., Roskamp KW; Department of Chemistry, University of California, Irvine, 1102 Natural Sciences 2, Irvine, CA, 92697-2025, USA., Martin RW; Department of Chemistry, University of California, Irvine, 1102 Natural Sciences 2, Irvine, CA, 92697-2025, USA.; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697-3900, USA.

Publikováno v: Chembiochem : a European journal of chemical biology [Chembiochem] 2021 Apr 16; Vol. 22 (8), pp. 1329-1346. Date of Electronic Publication: 2021 Feb 10.

Human γS-Crystallin-Copper Binding Helps Buffer against Aggregation Caused by Oxidative Damage.

Autor: Roskamp KW; Department of Chemistry, University of California, Irvine, California 92697-2025, United States., Azim S; Max Planck Institute for the Structure and Dynamics of Matter, Center for Free Electron Laser Science, Luruper Chaussee 149, Hamburg 22761, Germany., Kassier G; Max Planck Institute for the Structure and Dynamics of Matter, Center for Free Electron Laser Science, Luruper Chaussee 149, Hamburg 22761, Germany., Norton-Baker B; Department of Chemistry, University of California, Irvine, California 92697-2025, United States.; Max Planck Institute for the Structure and Dynamics of Matter, Center for Free Electron Laser Science, Luruper Chaussee 149, Hamburg 22761, Germany., Sprague-Piercy MA; Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, United States., Miller RJD; Max Planck Institute for the Structure and Dynamics of Matter, Center for Free Electron Laser Science, Luruper Chaussee 149, Hamburg 22761, Germany.; Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada., Martin RW; Department of Chemistry, University of California, Irvine, California 92697-2025, United States.; Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697-3900, United States.

Publikováno v: Biochemistry [Biochemistry] 2020 Jun 30; Vol. 59 (25), pp. 2371-2385. Date of Electronic Publication: 2020 Jun 12.