Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR

Autor: Xinshu Qin, Xingyu Wang, Ke Xu, Yi Zhang, Hongye Tian, Yinglei Li, Bangran Qi, Xingbin Yang

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 31, Iss , Pp 241-255 (2023)

Here, a method using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER) for miRNAs quantification was established. The strategy has two steps: (1) ligation of two DNA probes specifically hybridize to target

Externí odkaz: https://doaj.org/article/3987910751f747899c9dc19c372c8fc9

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR.

Autor: Qin X; College of Food Engineering and Nutritional Science, Shaanxi Normal University, No. 620 West Chang'an Avenue, Xi'an 710062, Shaanxi, China., Wang X; College of Food Engineering and Nutritional Science, Shaanxi Normal University, No. 620 West Chang'an Avenue, Xi'an 710062, Shaanxi, China., Xu K; Department of Joint Surgery, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an 710054, Shaanxi, China., Zhang Y; Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA., Tian H; College of Food Engineering and Nutritional Science, Shaanxi Normal University, No. 620 West Chang'an Avenue, Xi'an 710062, Shaanxi, China., Li Y; College of Food Engineering and Nutritional Science, Shaanxi Normal University, No. 620 West Chang'an Avenue, Xi'an 710062, Shaanxi, China., Qi B; College of Food Engineering and Nutritional Science, Shaanxi Normal University, No. 620 West Chang'an Avenue, Xi'an 710062, Shaanxi, China., Yang X; College of Food Engineering and Nutritional Science, Shaanxi Normal University, No. 620 West Chang'an Avenue, Xi'an 710062, Shaanxi, China.

Publikováno v: Molecular therapy. Nucleic acids [Mol Ther Nucleic Acids] 2022 Dec 27; Vol. 31, pp. 241-255. Date of Electronic Publication: 2022 Dec 27 (Print Publication: 2023).

Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR

Autor: Hui Wang, Jun Han, Weixiang Hong, Mai Zhang, Yuting Jia, Zhengping Li, Honghong Wang

Publikováno v: Chemical Communications. 57:10011-10014

A method based on mRNA-templated ligation of splice-junction anchored DNA probes followed by PCR amplification of the ligated product has been developed for multiplexed detection of mRNA splice variants with high sensitivity and specificity. The prop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::16e0d842f7f845e53a885d056b2d77f1
https://doi.org/10.1039/d1cc03033g

A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification.

Autor: Wu, Saiwei¹ (AUTHOR), Abdullah Al-Maskri, Abdu Ahmed² (AUTHOR), Li, Qun¹ (AUTHOR), Liu, Jiatong² (AUTHOR), Cai, Sheng² (AUTHOR)

Publikováno v: International Journal of Analytical Chemistry. 9/12/2023, p1-8. 8p.