Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Spencer C. Alford"'
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 13, Iss , Pp 144-153 (2018)
Aniridia is a rare congenital syndrome that is associated with reduced visual acuity and progressive loss of vision. Aniridia patients may also develop systemic health issues associated with defects in the pancreas, digestive, and central nervous sys
Externí odkaz:
https://doaj.org/article/a40bcde9810942e3b9480c0e874e6afe
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 13, Iss, Pp 144-153 (2018)
Molecular Therapy. Nucleic Acids
Molecular Therapy. Nucleic Acids
Aniridia is a rare congenital syndrome that is associated with reduced visual acuity and progressive loss of vision. Aniridia patients may also develop systemic health issues associated with defects in the pancreas, digestive, and central nervous sys
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4b380ced349e7c7ead69a0f47c947e0
https://doi.org/10.1016/j.omtn.2018.08.018
https://doi.org/10.1016/j.omtn.2018.08.018
Autor:
Cheryl H. Chang, Deepti Kannan, Sean A. Hunter, R Andres Parra Sperberg, Spencer C. Alford, Aaron C. Mitchell, Jennifer R. Cochran
Publikováno v:
ACS Chemical Biology. 13:66-72
Dysregulated activity of the protease matriptase is a key contributor to aggressive tumor growth, cancer metastasis, and osteoarthritis. Methods for the detection and quantification of matriptase activity and inhibition would be useful tools. To addr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::858ec02e1cc91dd550dcceff5041014c
https://doi.org/10.1021/acschembio.7b00715
https://doi.org/10.1021/acschembio.7b00715
Autor:
Thomas M. Baer, Daniel Herschlag, Bob Chen, Spencer C. Alford, Arvind Kannan, Fanny Sunden, Jennifer R. Cochran, Ivan Dimov, Sung Won Lim
Publikováno v:
Nature chemical biology
We describe a multi-purpose technology platform, termed μSCALE (Microcapillary Single Cell Analysis and Laser Extraction), that enables massively parallel, quantitative biochemical and biophysical measurements on millions of protein variants express
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a4b2cf2ac47719e0d01c0d128390187
https://doi.org/10.1038/nchembio.1978
https://doi.org/10.1038/nchembio.1978
Autor:
Jennifer Christie, Perry L. Howard, Connor O'Sullivan, Jake Gambling, Robert L. Chow, Marcus Pienaar, Spencer C. Alford, Philip E. B. Nickerson
Publikováno v:
Molecular and Cellular Biology. 35:3753-3767
ARS2 is a regulator of RNA polymerase II transcript processing through its role in the maturation of distinct nuclear cap-binding complex (CBC)-controlled RNA families. In this study, we examined ARS2 domain function in transcript processing. Structu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::734fd59306f141074168a31cdc5071ca
https://doi.org/10.1128/mcb.00272-15
https://doi.org/10.1128/mcb.00272-15
Autor:
Yidan Ding, Robert E. Campbell, Anne Marie Quinn, Thomas E. Hughes, Gary C. H. Mo, Yan Zhang, Spencer C. Alford, Jin Zhang, Paul Tewson, Yi Shen, Jiao Li, Issan Zhang, Dusica Maysinger, Jhon R. Enterina
Publikováno v:
Nature methods
We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76ae08e3353678380d4d412560d494f8
https://doi.org/10.1038/nmeth.3261
https://doi.org/10.1038/nmeth.3261
Publikováno v:
Biology of the Cell. 105:14-29
The discovery of naturally evolved fluorescent proteins and their subsequent tuning by protein engineering provided the basis for a large family of genetically encoded biosensors that report a variety of physicochemical processes occurring in living
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::030d053d2e310f1bd1c882b8b162fcd8
https://doi.org/10.1111/boc.201200054
https://doi.org/10.1111/boc.201200054
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1495
Protein toxin splicing mediated by split inteins can be used as a strategy for conditional cell ablation. The approach requires artificial fragmentation of a potent protein toxin and tethering each toxin fragment to a split intein fragment. The toxin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::20aeae1da9276441078fe52c12fd7b56
https://pubmed.ncbi.nlm.nih.gov/27714618
https://pubmed.ncbi.nlm.nih.gov/27714618
Publikováno v:
Methods in Molecular Biology ISBN: 9781493964499
Protein toxin splicing mediated by split inteins can be used as a strategy for conditional cell ablation. The approach requires artificial fragmentation of a potent protein toxin and tethering each toxin fragment to a split intein fragment. The toxin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::24fac195931d7e6b94abde2a4e7e02a4
https://doi.org/10.1007/978-1-4939-6451-2_13
https://doi.org/10.1007/978-1-4939-6451-2_13
Publikováno v:
ACS Synthetic Biology. 1:569-575
Dimerization-dependent fluorescent proteins (ddFP) are a recently introduced class of genetically encoded reporters that can be used for the detection of protein interactions in live cells. The progenitor of this class of tools was a red fluorescent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae17e7bb7787fe42b61382c652ea5397
https://doi.org/10.1021/sb300050j
https://doi.org/10.1021/sb300050j