Výsledky vyhledávání - "Spaink Herman P"

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Akademický článek

Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda

Autor: Bloemberg Guido V, Ordas Anita, Stockhammer Oliver W, van Soest Joost J, Spaink Herman P, Meijer Annemarie H

Publikováno v: BMC Immunology, Vol 12, Iss 1, p 58 (2011)

Abstract Background The zebrafish embryo is an important in vivo model to study the host innate immune response towards microbial infection. In most zebrafish infectious disease models, infection is achieved by micro-injection of bacteria into the em

Externí odkaz: https://doaj.org/article/f4ac5a27977944ad98259a0df3717e62

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Akademický článek

Rapid screening of innate immune gene expression in zebrafish using reverse transcription - multiplex ligation-dependent probe amplification

Autor: Spaink Herman P, Butler Derek, van Gils Walter, Rotman Janneke, Meijer Annemarie H

Publikováno v: BMC Research Notes, Vol 4, Iss 1, p 196 (2011)

Abstract Background With the zebrafish increasingly being used in immunology and infectious disease research, there is a need for efficient molecular tools to evaluate immune gene expression in this model species. RT-MLPA (reverse transcription - mul

Externí odkaz: https://doaj.org/article/40407d99c358432c9b64174446300d50

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Akademický článek

The epigenetic regulator Histone Deacetylase 1 promotes transcription of a core neurogenic programme in zebrafish embryos

Autor: Georgiou Aristophanes S, Harrison Michael RM, Spaink Herman P, Cunliffe Vincent T

Publikováno v: BMC Genomics, Vol 12, Iss 1, p 24 (2011)

Abstract Background The epigenetic regulator Histone Deacetylase 1 (Hdac1) is required for specification and patterning of neurones and myelinating glia during development of the vertebrate central nervous system (CNS). This co-ordinating function fo

Externí odkaz: https://doaj.org/article/d01e295648aa4823b7f6b14f44cf83a3

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Akademický článek

Integrating heterogeneous sequence information for transcriptome-wide microarray design; a Zebrafish example

Autor: de Leeuw Wim C, de Jong Mark, Rauwerda Han, Spaink Herman P, Breit Timo M

Publikováno v: BMC Research Notes, Vol 3, Iss 1, p 192 (2010)

Abstract Background A complete gene-expression microarray should preferably detect all genomic sequences that can be expressed as RNA in an organism, i.e. the transcriptome. However, our knowledge of a transcriptome of any organism still is incomplet

Externí odkaz: https://doaj.org/article/1e13168543aa44a289912a136c2dab03

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Akademický článek

Swimming suppresses hepatic vitellogenesis in European female silver eels as shown by expression of the estrogen receptor 1, vitellogenin1 and vitellogenin2 in the liver

Autor: Nieveen Maaike C, Schnabel Denhi, Palstra Arjan P, Spaink Herman P, van den Thillart Guido

Publikováno v: Reproductive Biology and Endocrinology, Vol 8, Iss 1, p 27 (2010)

Abstract Background When European silver eels (Anguilla anguilla) venture into the Atlantic Ocean for their 6,000 km semelparous spawning run to the Sargasso Sea, they are still in a prepubertal stage. Further sexual development appears to be blocked

Externí odkaz: https://doaj.org/article/10bff36eca2841b997005d1c6a144bf3

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Akademický článek

RNA isolation method for single embryo transcriptome analysis in zebrafish

Autor: Spaink Herman P, Verkooijen Jurgo, Bruning Oskar, Rauwerda Han, de Jong Mark, Breit Timo M

Publikováno v: BMC Research Notes, Vol 3, Iss 1, p 73 (2010)

Abstract Background Transcriptome analysis during embryogenesis usually requires pooling of embryos to obtain sufficient RNA. Hence, the measured levels of gene-expression represent the average mRNA levels of pooled samples and the biological variati

Externí odkaz: https://doaj.org/article/e82594045eb648efac4ee9c5d301fe3f

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