Zobrazeno 1 - 10
of 180
pro vyhledávání: '"SpCas9"'
Publikováno v:
Journal of Translational Medicine, Vol 22, Iss 1, Pp 1-29 (2024)
Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 system, a groundbreaking innovation in genetic engineering, has revolutionized our approach to surmounting complex diseases, culminating in CASGEVY™ approved for si
Externí odkaz:
https://doaj.org/article/aafc679c11514c4ba333714e9074b592
Autor:
Zhi-Xue Yang, Ya-Wen Fu, Juan-Juan Zhao, Feng Zhang, Si-Ang Li, Mei Zhao, Wei Wen, Lei Zhang, Tao Cheng, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1206-1220 (2023)
A series of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) systems have been engineered for genome editing. The most widely used Cas9 is SpCas9 from Streptococcus pyogenes and SaCas9 from Staphyl
Externí odkaz:
https://doaj.org/article/dd9a845d89064b8db6bec0665d9eb342
Autor:
Khaled Essawi, Waleed Hakami, Muhammad Behroz Naeem Khan, Reid Martin, Jing Zeng, Rebecca Chu, Naoya Uchida, Aylin C. Bonifacino, Allen E. Krouse, Nathaniel S. Linde, Robert E. Donahue, Gerd A. Blobel, Ulrike Gerdemann, Leslie S. Kean, Stacy A. Maitland, Scot A. Wolfe, Jean-Yves Metais, Stephen Gottschalk, Daniel E. Bauer, John F. Tisdale, Selami Demirci
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 29, Iss , Pp 483-493 (2023)
CRISPR-Cas9-based therapeutic genome editing approaches hold promise to cure a variety of human diseases. Recent findings demonstrate pre-existing immunity for the commonly used Cas orthologs from Streptococcus pyogenes (SpCas9) and Staphylococcus au
Externí odkaz:
https://doaj.org/article/186bb2a612c54f99a14f07e9473590ec
Autor:
Shilpi Agrawal, Made Harumi Padmaswari, Abbey L. Stokes, Daniel Maxenberger, Morgan Reese, Adila Khalil, Christopher E. Nelson
Publikováno v:
Biomedicines, Vol 12, Iss 6, p 1226 (2024)
The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely. However, w
Externí odkaz:
https://doaj.org/article/2f2c2d43055c4acc9c6058f06ddb984c
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 34, Iss , Pp 102072- (2023)
Paired SpCas9 nickases (SpCas9n) are an effective strategy to reduce off-target effect in genome editing. However, this approach is not efficient with 3′-overhanging ends, limiting its applications. In order to expand the utility of paired SpCas9n
Externí odkaz:
https://doaj.org/article/8c880b1b54f84caeabaf083b01abb9ed
Autor:
Jana Ordon, Niklas Kiel, Dieter Becker, Carola Kretschmer, Paul Schulze-Lefert, Johannes Stuttmann
Publikováno v:
Plant Methods, Vol 19, Iss 1, Pp 1-11 (2023)
Abstract Background In plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus pyogenes (SpCas9) predominantly induce small insertions or deletions at target sites. This can be used for inactivation of protein-coding genes by frame
Externí odkaz:
https://doaj.org/article/98f292c9508346c7a37ee1dd8b0dcf16
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Akademický článek
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Autor:
Yue Yang, Donghua Li, Fen Wan, Bohong Chen, Guanglan Wu, Feng Li, Yanliang Ren, Puping Liang, Jian Wan, Zhou Songyang
Publikováno v:
Cells, Vol 11, Iss 22, p 3574 (2022)
Genome editing tools based on CRISPR–Cas systems can repair genetic mutations in situ; however, off-target effects and DNA damage lesions that result from genome editing remain major roadblocks to its full clinical implementation. Protein and chemi
Externí odkaz:
https://doaj.org/article/21a62802e00b450ba7ba2eb2f652e8da