A ribosome-associated chaperone enables substrate triage in a cotranslational protein targeting complex

Autor: Hao-Hsuan Hsieh, Jae Ho Lee, Sowmya Chandrasekar, Shu-ou Shan

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-20 (2020)

Biochemistry combined with biophysical measurements and mathematical modeling offer insight into the mechanism by which the cotranslational chaperone, nascent polypeptide-associated complex (NAC), modulates substrate selection by signal recognition p

Externí odkaz: https://doaj.org/article/1adc0df7a6714d4c96bfb07681a11801

Receptor compaction and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER

Autor: Yu-Hsien Hwang Fu, Hao-Hsuan Hsieh, Nenad Ban, Daniel Boehringer, Ahmad Jomaa, Sowmya Chandrasekar, Shu-ou Shan, Simone Mattei, SangYoon Chung, Xuemeng Sun, Shimon Weiss, Ruilin Qian, Jae Ho Lee, Xiaotian Bi

Publikováno v: Science Advances
Science advances, vol 7, iss 21
Science Advances, 7 (21)

The conserved signal recognition particle (SRP) cotranslationally delivers ~30% of the proteome to the eukaryotic endoplasmic reticulum (ER). The molecular mechanism by which eukaryotic SRP transitions from cargo recognition in the cytosol to protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::352304ac5a375070e5db54ccedae8757
http://europepmc.org/articles/PMC8139590

A ribosome-associated chaperone enables substrate triage in a cotranslational protein targeting complex

Autor: Sowmya Chandrasekar, Jae Ho Lee, Shu-ou Shan, Hao-Hsuan Hsieh

Publikováno v: Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-20 (2020)

Protein biogenesis is essential in all cells and initiates when a nascent polypeptide emerges from the ribosome exit tunnel, where multiple ribosome-associated protein biogenesis factors (RPBs) direct nascent proteins to distinct fates. How distinct

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6eebf4746579f1e6aff954a5fd69b0f1
https://pubmed.ncbi.nlm.nih.gov/33203865

Sequential activation of human signal recognition particle by the ribosome and signal sequence drives efficient protein targeting

Autor: Jae Ho Lee, SangYoon Chung, Sowmya Chandrasekar, Shimon Weiss, Shu-ou Shan, Demi Liu, Yu-Hsien Hwang Fu

Publikováno v: Lee, Jae Ho; Chandrasekar, Sowmya; Chung, SangYoon; Hwang Fu, Yu-Hsien; Liu, Demi; Weiss, Shimon; et al.(2018). Sequential activation of human signal recognition particle by the ribosome and signal sequence drives efficient protein targeting.. Proceedings of the National Academy of Sciences of the United States of America, 115(24), E5487-E5496. doi: 10.1073/pnas.1802252115. UCLA: Retrieved from: http://www.escholarship.org/uc/item/7bq0r64v
Proceedings of the National Academy of Sciences of the United States of America, vol 115, iss 24

Signal recognition particle (SRP) is a universally conserved targeting machine that mediates the targeted delivery of ∼30% of the proteome. The molecular mechanism by which eukaryotic SRP achieves efficient and selective protein targeting remains e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b58eeb58dfe4f27d496d62c196bfa901
http://www.escholarship.org/uc/item/7bq0r64v

Mechanistic Studies on the Mononuclear ZnII-Containing Metallo-β-lactamase ImiS from Aeromonas sobria

Autor: Christine E. Hajdin, Sowmya Chandrasekar, Narayan Sharma, Michael W. Crowder, Ke-Wu Yang, Brian Bennett

Publikováno v: Biochemistry. 45:10729-10738

Bacterial resistance to antibiotics is a growing clinical concern (1, 2). Zn(II)-containing β-lactamases (metallo-β-lactamases, MβL’s) contain 1–2 moles of Zn(II) per mole of enzyme, hydrolyze all known cephalosporins, carbapenems and penicill

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::571bdd1384c5f5e3e858a4605ec1fb44
https://doi.org/10.1021/bi060893t