Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques

Autor: Saber Khelaifia, Pilliol Virginie, Souad Belkacemi, Herve Tassery, Elodie Terrer, Gérard Aboudharam

Publikováno v: Microorganisms, Vol 11, Iss 4, p 836 (2023)

Recent years have been marked by a paradigm shift in the study of the human microbiota, with a re-emergence of culture-dependent approaches. Numerous studies have been devoted to the human microbiota, while studies on the oral microbiota still remain

Externí odkaz: https://doaj.org/article/1f1eb25b37d24491ab9e948c36f01874

A review of

Autor: Souad, Belkacemi, Maryam, Tidjani Alou, Saber, Khelaifia, Didier, Raoult

Publikováno v: Journal of medical microbiology. 70(7)

To date, the axenic culture of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::99369b564a74a5a52e90b83f788055fa
https://pubmed.ncbi.nlm.nih.gov/34328411

Description of Prevotella rectalis sp. nov., a new bacterium isolated from human rectum

Autor: Pierre-Edouard Fournier, Saber Khelaifia, Cheikh Ibrahima Lo, Florence Fenollar, Didier Raoult, Souad Belkacemi

Publikováno v: New Microbes and New Infections
New Microbes and New Infections, 2020, 36, pp.100703. ⟨10.1016/j.nmni.2020.100703⟩
New Microbes and New Infections, Wiley Online Library 2020, 36, pp.100703. ⟨10.1016/j.nmni.2020.100703⟩
New Microbes and New Infections, Vol 36, Iss, Pp 100703-(2020)

International audience; Using a taxonogenomics method, we describe here a Gram-negative bacterium named Prevotella rectalis sp. nov., strain Marseille-P4334 T (= CSUR P4334) isolated from the rectum. Strain Marseille-P4334 T has a genome that measure

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::631d84a3556c25efac9cdf1b034d7a1f
https://hal-amu.archives-ouvertes.fr/hal-03142058

Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Treponema Culture and Identification from the Oral Cavity

Autor: Didier Raoult, Anthony Levasseur, Saber Khelaifia, Souad Belkacemi, Jacques Yaacoub Bou Khalil, Akiko Hisada, Aurelia Caputo, Jean-Christophe Lagier, Anthony Fontanini, Yusuke Ominami

Publikováno v: Journal of Clinical Microbiology
Journal of Clinical Microbiology, American Society for Microbiology, 2019, 57 (10), ⟨10.1128/JCM.00517-19⟩
Journal of Clinical Microbiology, 2019, 57 (10), ⟨10.1128/JCM.00517-19⟩

We present here a new passive-filtration-based culture device combined with rapid identification with a new electron microscope (Hitachi TM4000) for the detection and culture of Treponema species from the human oral cavity. Of the 44 oral samples cul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90ae723c68a791a92e67a34e60d4717d
https://hal-amu.archives-ouvertes.fr/hal-02457977

Peri-implantitis-associated methanogens: a preliminary report

Autor: Anthony Mazel, Michel Drancourt, Delphine Tardivo, Giancarlo Bianca, Souad Belkacemi, Patrick Tavitian, Grégory Stephan, Elodie Terrer, Gérard Aboudharam

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2018, 8, pp.9447. ⟨10.1038/s41598-018-27862-8⟩
Scientific Reports, Vol 8, Iss 1, Pp 1-6 (2018)
Scientific Reports, 2018, 8, pp.9447. ⟨10.1038/s41598-018-27862-8⟩
Scientific Reports, 2018, 8 (1), pp.9447. ⟨10.1038/s41598-018-27862-8⟩

Methanogens have already been described in periodontitis but not in peri-implantitis. Thirty peri-implantitis samples and 28 control samples were collected in 28 consenting peri-implantitis patients. PCR-sequencing of the 16S rRNA gene was used as a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80fdf39be49bff1257bb9d1301f31d53
https://hal.archives-ouvertes.fr/hal-01858891/document