The functionality of a therapeutic antibody candidate restored by a single mutation from proline to threonine in the variable region

Autor: Marie Hautiere, Irene Maffucci, Narciso Costa, Amaury Herbet, Sosthene Essono, Séverine Padiolleau-Lefevre, Didier Boquet

Publikováno v: Human Vaccines & Immunotherapeutics, Vol 19, Iss 3 (2023)

ABSTRACTmAbs play an essential role in the therapeutic arsenal. Our laboratory has patented the Rendomab-B49 mAb targeting the endothelin B receptor (ETB). This G protein-coupled receptor plays a driving role in the progression of numerous cancers. W

Externí odkaz: https://doaj.org/article/5457d5c48a09474cae6ab11171ce34f8

Overlapping synthetic peptides encoding TPD52 as breast cancer vaccine in mice: Prolonged survival

Autor: James B. Whitney, Sandra J. Lee, Sosthène Essono, Victor G. Kramer, Karen S. Anderson, Saied Mirshahidi, Glenn Dranoff, Ruth M. Ruprecht

Publikováno v: Vaccine. 27:1825-1833

Peptide-based vaccines, one of several anti-tumor immunization strategies currently under investigation, can elicit both MHC Class I-restricted (CD8(+)) and Class II-restricted (CD4(+)) responses. However, the need to identify specific T-cell epitope

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07568927b8ccabdeeddd075f8d1e4e24
https://doi.org/10.1016/j.vaccine.2009.01.089

A general method allowing the design of oligonucleotide primers to amplify the variable regions from immunoglobulin cDNA

Autor: Didier Boquet, Sosthène Essono, Jacques Grassi, Yveline Frobert, Christophe Créminon

Publikováno v: Journal of Immunological Methods. 279:251-266

The amplification of variable regions of immunoglobulins by reverse transcription polymerase chain reaction (RT-PCR) has become an invaluable technique either for the cloning of monoclonal antibodies (mAbs), or for the building of single-chain fragme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff1c69a17397e163f81e8cfa97da33df
https://doi.org/10.1016/s0022-1759(03)00242-4

Peptide mass-assisted antibody cloning strategy for accurate characterization of potential therapeutic monoclonal antibodies against neurodegenerative diseases

Autor: Gilles Clément, Sosthène Essono, Jacques Grassi, Christophe Créminon, Séverine Padiolleau-Lefèvre, Didier Boquet

Publikováno v: Protein Engineering, Design and Selection
Protein Engineering, Design and Selection, Oxford University Press (OUP), 2010, 23, pp.203-210. ⟨10.1093/protein/gzp071⟩
Protein Engineering, Design and Selection, 2010, 23, pp.203-210. ⟨10.1093/protein/gzp071⟩
Protein Engineering, Design and Selection, Oxford University Press (OUP), 2010, 23, pp.203-210

International audience; The development of therapeutic recombinant antibodies involves accurate characterization of immunoglobulin variable light (VL) and heavy (VH) chains. However, it has been reported that the use of subgroup or isotype-specific p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5a98c7026c5b1d006dc7a66cb420814
https://hal.archives-ouvertes.fr/hal-00468465

Structural insights into AChE inhibition by monoclonal antibodies

Autor: Ludovic, Renault, Sosthène, Essono, Marianick, Juin, Didier, Boquet, Jacques, Grassi, Yves, Bourne, Pascale, Marchot

Publikováno v: Chemico-biological interactions.

The target sites of three inhibitory monoclonal antibodies, Elec403, 408 and 410, on eel AChE have been defined previously. Elec403 and 410 are directed toward distinct but overlapping epitopes at the enzyme peripheral site, while Elec408 binds to a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c62b050acff9b77c3f15af5e7f41d79d
https://pubmed.ncbi.nlm.nih.gov/16429535