Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer

Autor: Agathe Couturier, Chloé Virolle, Kelly Goldlust, Annick Berne-Dedieu, Audrey Reuter, Sophie Nolivos, Yoshiharu Yamaichi, Sarah Bigot, Christian Lesterlin

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)

Conjugation is a contact-dependent mechanism for the transfer of plasmid DNA between bacterial cells. Here, Couturier et al. use live-cell microscopy to visualise the intracellular dynamics of conjugation in real time, revealing a molecular strategy

Externí odkaz: https://doaj.org/article/da4fc445eeb0466cab6f0b4efad27857

Real time visualisation of conjugation reveals the molecular strategy evolved by the conjugative F plasmid to ensure the sequential production of plasmid factors during establishment in the new host cell

Autor: Agathe Couturier, Chloé Virolle, Kelly Goldlust, Annick Berne-Dedieu, Audrey Reuter, Sophie Nolivos, Yoshiharu Yamaichi, Sarah Bigot, Christian Lesterlin

DNA conjugation is a contact-dependent horizontal gene transfer mechanism responsible for disseminating drug resistance among bacterial species. Conjugation remains poorly characterised at the cellular scale, particularly regarding the reactions occu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::45e772480423a1b21cf1639c60063e00
https://doi.org/10.1101/2022.09.06.506729

AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled Escherichia coli

Autor: Mélanie Bonnecaze, Joachim Allouche, Sophie Nolivos, Cécile Courrèges, Régis Grimaud, Delphine Flahaut

Publikováno v: Chemical Communications
Chemical Communications, Royal Society of Chemistry, 2021, 57 (44), pp.5446-5449. ⟨10.1039/d1cc01211h⟩

International audience; A chemical fingerprint of theEscherichia colicell surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight Secon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3023d4a5180563facb91ae87788adb2
https://hal-univ-pau.archives-ouvertes.fr/hal-03265407

AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled

Autor: Cécile, Courrèges, Mélanie, Bonnecaze, Delphine, Flahaut, Sophie, Nolivos, Régis, Grimaud, Joachim, Allouche

Publikováno v: Chemical communications (Cambridge, England). 57(44)

A chemical fingerprint of the Escherichia coli cell surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight Secondary Ion Mass Spectros

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b9211fe1424cada222f96c4c27d950fb
https://pubmed.ncbi.nlm.nih.gov/33950059

Co-evolution of segregation guide DNA motifs and the FtsK translocase in bacteria: identification of the atypical Lactococcus lactis KOPS motif

Autor: Michèle Coddeville, Meriem El Karoui, François Cornet, Pascal Le Bourgeois, Fabrice Touzain, Philippe Rousseau, Carine Pages, Sophie Nolivos

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2012, 40 (12), pp.5535-5545. ⟨10.1093/nar/gks171⟩
Nucleic Acids Research 12 (40), 5535-5545. (2012)
Nolivos, S, Touzain, F, Pages, C, Coddeville, M, Rousseau, P, El Karoui, M, Le Bourgeois, P & Cornet, F 2012, ' Co-evolution of segregation guide DNA motifs and the FtsK translocase in bacteria: identification of the atypical Lactococcus lactis KOPS motif ', Nucleic Acids Research, vol. 40, no. 12, pp. 5535-5545 . https://doi.org/10.1093/nar/gks171

Bacteria use the global bipolarization of their chromosomes into replichores to control the dynamics and segregation of their genome during the cell cycle. This involves the control of protein activities by recognition of specific short DNA motifs wh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72e0c646c5b95cfaabaf42961940ab47
https://hal.archives-ouvertes.fr/hal-01003421