Výsledky vyhledávání - "Sophie Nallet"

Akademický článek

Large‐scale 13C‐flux analysis reveals distinct transcriptional control of respiratory and fermentative metabolism in Escherichia coli

Autor: Bart R B Haverkorn van Rijsewijk, Annik Nanchen, Sophie Nallet, Roelco J Kleijn, Uwe Sauer

Publikováno v: Molecular Systems Biology, Vol 7, Iss 1, Pp 1-12 (2011)

Abstract Despite our increasing topological knowledge on regulation networks in model bacteria, it is largely unknown which of the many co‐occurring regulatory events actually control metabolic function and the distribution of intracellular fluxes.

Externí odkaz: https://doaj.org/article/c6493d8009cf4e6dbce9e4ac23543f7e

Zobrazit plný text záznamu

Plný text ve formátu HTML

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N -Propionyl Groups

Autor: Zuzana Kadlecova, Lucia Baldi, David L. Hacker, Florian M. Wurm, Sophie Nallet, Harm-Anton Klok

Publikováno v: Macromolecular Bioscience. 12:628-636

Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI "Max") regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b405bd880ab82184e977f61b5a2b9c6b
https://doi.org/10.1002/mabi.201100404

Zobrazit plný text záznamu

Structural Analysis of Intact Monoclonal Antibodies by Electron Transfer Dissociation Mass Spectrometry

Autor: Carsten Stoermer, Yury O. Tsybin, Markus Luebeck, Luca Fornelli, Sophie Nallet, Julien Parra, Ralf Hartmer, Florian M. Wurm

Publikováno v: Analytical Chemistry. 83:8919-8927

Improving qualitative and quantitative characterization of monoclonal antibodies is essential, because of their increasing popularity as therapeutic drug targets. Electron transfer dissociation (ETD)-based top-down mass spectrometry (MS) is the metho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14f831bf6ca39c81d1177ef5dca59b5d
https://doi.org/10.1021/ac201293m

Zobrazit plný text záznamu

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

Autor: Nicole Westerfeld, Sophie Nallet, Lucia Baldi, Iwo König, David L. Hacker, Mario Amacker, Christiane Zaborosch, Rinaldo Zurbriggen, Florian M. Wurm

Publikováno v: Vaccine. 27:6415-6419

Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d907f1e3bec0fa743c1a0d1bc578dbb
https://doi.org/10.1016/j.vaccine.2009.06.019

Zobrazit plný text záznamu

Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups

Autor: Zuzana, Kadlecova, Sophie, Nallet, David L, Hacker, Lucia, Baldi, Harm-Anton, Klok, Florian M, Wurm

Publikováno v: Macromolecular bioscience. 12(5)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fe8618c731e290d85f6584651ea0821d
https://pubmed.ncbi.nlm.nih.gov/22411776

Zobrazit plný text záznamu

Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance

Autor: Sophie Nallet, Lucia Baldi, Florian M. Wurm, Harm-Anton Klok, Zuzana Kadlecova

Publikováno v: Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009 ISBN: 9789400708839

Transient gene expression (TGE) allows production of virtually any recombinant protein (r-protein) in mammalian cells. Its flexibility, speed, scalability, and cost-effectiveness have been widely demonstrated. However, good manufacturing practices (G

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3ed035a07dd1de9661a46c52139de74f
https://doi.org/10.1007/978-94-007-0884-6_13

Zobrazit plný text záznamu

Large-scale 13C-flux analysis reveals distinct transcriptional control of respiratory and fermentative metabolism in Escherichia coli

Autor: Uwe Sauer, Bart R. B. Haverkorn van Rijsewijk, Sophie Nallet, Roelco J. Kleijn, Annik Nanchen

Publikováno v: Molecular Systems Biology
Molecular Systems Biology, 7 (1)

The authors analyze the role transcription plays in regulating bacterial metabolic flux. Of 91 transcriptional regulators studied, 2/3 affect absolute fluxes, but only a small number of regulators control the partitioning of flux between different me

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4cad9ec9ebafbd0378b1315bd1d77c7

Zobrazit plný text záznamu

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells

Autor: Simone Schmitt, Yury O. Tsybin, Sophie Nallet, Lucia Baldi, Julien Parra, Luca Fornelli, Florian M. Wurm

In this study, a recombinant monoclonal IgG antibody was produced by transient gene expression (TGE) in suspension-adapted HEK-293E cells. The objective of the study was to determine the variation in recombinant IgG yield and glycosylation in ten ind

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f102e42b86d272d13a21129ebb254520
https://infoscience.epfl.ch/record/176728

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání