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pro vyhledávání: '"Sophie Destrau"'
Autor:
James E Lynch, John M. Roach, Sophie Destrau, Toby T. Hecht, J.Daniel Coffman, Thomas F. Ouellette, Steven L. Giardina, Timothy F. Ouellette, Jianwei Zhu
Publikováno v:
Protein Expression and Purification. 30:156-166
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by h