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Publikováno v:
Analytical Biochemistry. 367:167-172
A spectrophotometric assay to determine peptide transport has been developed. Using two chromogenic peptide mimetics, L-phenylalanyl-L-2-sulfanilylglycine (PSG) and L-phenylalanyl-L-3-thiaphenylalanine (PSP), the peptide transport patterns in individ
Publikováno v:
Journal of microbiology and biotechnology. 25(10)
Peptide assimilation in Helicobacter pylori necessitates a coordinated working of the peptide transport systems (PepTs) and aminopeptidase (PepA). We found that H. pylori hydrolyzes two detector peptides, L-phenylalanyl-L-3-thiaphenylalanine (PSP) an
Publikováno v:
Journal of microbiology and biotechnology. 21(5)
An acid phosphatase (HppA) activated by NH4Cl was purified 192- and 34-fold from the periplasmic and membrane fractions of Helicobacter pylori, respectively. SDS-polyacrylamide gel electrophoresis revealed that HppA from the latter appears to be seve
Publikováno v:
Journal of microbiology and biotechnology. 19(12)
Purified Helicobacter pylori urease displayed a sigmoid curve in the plot of velocity versus [S] at urea concentrations less than 0.1 mM. Under conditions where preservatives, glycerol, or polyethylene glycol (PEG), were added to the enzyme reaction,