Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Sonia Advani"'
Publikováno v:
Biochemical and Biophysical Research Communications. 402:177-179
Recently determined crystal structures of type II restriction endonucleases have produced a plethora of information on the basis for target site sequence selectivity. The positioning and role of metal ions in DNA recognition sites might reflect impor
Autor:
Sonia Advani, Ajay Kumar
Publikováno v:
Nucleosides and Nucleotides. 11:999-1002
A method for introducing a -SH group at the 5′-end of the oligonucleotide using S-Trityl-L-Cysteine has been developed. Carbonyl diimidazole was used for activating the 5′-OH group.
Autor:
Pradeep Kumar Burma, Sudipta Datta, Suma Chakravarthy, Simran Bhullar, Taru Gautam, Deepak Pental, Sonia Advani
Publikováno v:
Plant biotechnology journal. 5(6)
Summary The cauliflower mosaic virus 35S (35S) promoter is used extensively for transgene expression in plants. The promoter has been delineated into different subdomains based on deletion analysis and gain-of-function studies. However, cis-elements
Autor:
Deepak Pental, Pradeep Kumar Burma, Suma Chakravarthy, Sudipta Datta, Sonia Advani, Simran Bhullar
Publikováno v:
Plant physiology. 132(2)
The cauliflower mosaic virus 35S (35S) promoter has been extensively used for the constitutive expression of transgenes in dicotyledonous plants. The repetitive use of the same promoter is known to induce transgene inactivation due to promoter homolo
Autor:
Sonia Advani, Kunal B. Roy
Publikováno v:
Biochemical and biophysical research communications. 279(1)
Biochemical properties of Type II restriction enzyme Ban I were characterized. Kinetic parameters were evaluated and an enhancement of rate was observed when the recognition site was located in a more central position in the substrate, suggesting tha