A systematic enhancer screen using lentivector transgenesis identifies conserved and non-conserved functional elements at the Olig1 and Olig2 locus.

Autor: Marc Friedli, Isabelle Barde, Mélanie Arcangeli, Sonia Verp, Alexandra Quazzola, Jozsef Zakany, Nathalie Lin-Marq, Daniel Robyr, Catia Attanasio, François Spitz, Denis Duboule, Didier Trono, Stylianos E Antonarakis

Publikováno v: PLoS ONE, Vol 5, Iss 12, p e15741 (2010)

Finding sequences that control expression of genes is central to understanding genome function. Previous studies have used evolutionary conservation as an indicator of regulatory potential. Here, we present a method for the unbiased in vivo screen of

Externí odkaz: https://doaj.org/article/d4dead86d0a8437c9a8deaede79cae00

The KZFP/KAP1 system controls transposable elements-embedded regulatory sequences in adult T cells

Autor: Didier Trono, Julien Duc, Laia Simó-Riudalbas, Flavia Marzetta, Evarist Planet, Sonia Verp, Priscilla Turelli

Transposable elements-embedded regulatory sequences (TEeRS) are subjected to early embryonic repression through sequence-specific recruitment of KRAB zinc finger proteins (KZFPs), their cofactor KAP1/TRIM28 and associated chromatin modifiers. This mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c44ab98e8f7c402f0dfff1680775c17
https://doi.org/10.1101/523597

Measuring In Vivo Protein Half-Life

Autor: Didier Trono, Sandra Offner, Karolina Bojkowska, Isabelle Barde, Sonia Verp, Christian Heinis, Francesca R. Santoni de Sio, Kai Johnsson

Publikováno v: Chemistry & biology

SummaryProtein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0210aa62bad2de191e5e77d4d97c4e52
https://doi.org/10.1016/j.chembiol.2011.03.014

KAP1 controls endogenous retroviruses in embryonic stem cells

Autor: Helen M. Rowe, Séverine Reynard, Julien Marquis, Tugce Aktas, Daniel Mesnard, Daniel B. Constam, François Spitz, Sonia Verp, Johan Jakobsson, Didier Trono, Hillary Layard-Liesching, Jacques Rougemont, Pierre V Maillard

Publikováno v: Nature. 463:237-240

More than forty per cent of the mammalian genome is derived from retroelements, of which about one-quarter are endogenous retroviruses (ERVs). Some are still active, notably in mice the highly polymorphic early transposon (ETn)/MusD and intracisterna

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62a9c4fc960fd28bee0eda04d262ba68
https://doi.org/10.1038/nature08674

Rescue of a severe mouse model for spinal muscular atrophy by U7 snRNA-mediated splicing modulation

Autor: Judith Trüb, Sonia Verp, Rachel Nlend Nlend, Isabelle Barde, Kathrin Meyer, Didier Trono, Daniel Schümperli, Marc-David Ruepp, Julien Marquis, Hans Imboden

Publikováno v: HUMAN MOLECULAR GENETICS
Meyer, Kathrin; Marquis, Julien; Trüb, Judith; Nlend Nlend, Rachel; Verp, Sonia; Ruepp, Marc-David; Imboden, Hans; Barde, Isabelle; Trono, Didier; Schümperli, Daniel (2009). Rescue of a severe mouse model for spinal muscular atrophy by U7 snRNA-mediated splicing modulation. Human molecular genetics, 18(3), pp. 546-555. Oxford: Oxford University Press 10.1093/hmg/ddn382

In spinal muscular atrophy (SMA), the leading genetic cause of early childhood death, the survival motor neuron 1 gene (SMN1) is deleted or inactivated. The nearly identical SMN2 gene has a silent mutation that impairs the utilization of exon 7 and t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef0cb110afcc1a35e3ef091be23fb9f9
http://hmg.oxfordjournals.org/content/18/3/546.long