CAR-T cell expansion platforms yield distinct T cell differentiation states.

Autor: Song HW; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Prochazkova M; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Shao L; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Traynor R; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Underwood S; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Black M; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Fellowes V; Center for Immuno-Oncology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Shi R; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Pouzolles M; Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Chou HC; Genomics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Cheuk AT; Genomics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Taylor N; Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Jin P; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Somerville RP; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Stroncek DF; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA., Khan J; Genomics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Highfill SL; Department of Transfusion Medicine, Center for Cellular Engineering, National Institutes of Health, Bethesda, MD, USA. Electronic address: steven.highfill@nih.gov.

Publikováno v: Cytotherapy [Cytotherapy] 2024 Jul; Vol. 26 (7), pp. 757-768. Date of Electronic Publication: 2024 Mar 12.

Deciphering the importance of culture pH on CD22 CAR T-cells characteristics.

Autor: Prochazkova M; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Dreyzin A; Center for Cancer and Blood Disorders, Children's National Hospital, Washington, DC, USA., Shao L; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Garces P; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Cai Y; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Shi R; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Pelayo A; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Kim YS; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Pham V; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Frodigh SE; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Fenton S; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Karangwa C; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Su Y; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Martin K; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Zhang N; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Highfill SL; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Somerville RP; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Shah NN; Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Stroncek DF; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA., Jin P; Center for Cellular Engineering, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA. pjin@mail.cc.nih.gov.

Publikováno v: Journal of translational medicine [J Transl Med] 2024 Apr 24; Vol. 22 (1), pp. 384. Date of Electronic Publication: 2024 Apr 24.

Manufacture of CD22 CAR T cells following positive versus negative selection results in distinct cytokine secretion profiles and γδ T cell output.

Autor: Song HW; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Benzaoui M; Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.; Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS, Montpellier, France., Dwivedi A; Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Underwood S; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Shao L; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Achar S; Laboratory of Integrative Cancer Immunology, NCI, Bethesda, MD, USA., Posarac V; STEMCELL Technologies, Vancouver, Canada., Remley VA; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Prochazkova M; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Cai Y; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Jin P; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Somerville RP; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Stroncek DF; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA., Altan-Bonnet G; Laboratory of Integrative Cancer Immunology, NCI, Bethesda, MD, USA., Shah NN; Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Chien CD; Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Taylor N; Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.; Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS, Montpellier, France., Highfill SL; Center for Cellular Engineering, Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD, USA.

Publikováno v: Molecular therapy. Methods & clinical development [Mol Ther Methods Clin Dev] 2023 Dec 11; Vol. 32 (1), pp. 101171. Date of Electronic Publication: 2023 Dec 11 (Print Publication: 2024).

Genome-wide profiling of retroviral DNA integration and its effect on clinical pre-infusion CAR T-cell products.

Autor: Shao L; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Shi R; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Zhao Y; Division of Cancer Treatment and Diagnosis, Biometric Research Program, National Cancer Institute, Rockville, MD, 90033, USA., Liu H; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Lu A; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Ma J; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Cai Y; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Fuksenko T; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Pelayo A; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Shah NN; Pediatric Oncology Branch, Center for Cancer Research, NCI, NIH, Bethesda, MD, 20892, USA., Kochenderfer JN; Surgery Branch, Center for Cancer Research, NCI, NIH, Bethesda, MD, 20892, USA., Norberg SM; Genitourinary Malignancies Branch Cellular Therapy program, Cancer Immunotherapy Program, NCI, NIH, Bethesda, MD, 20892, USA., Hinrichs C; Genitourinary Malignancies Branch Cellular Therapy program, Cancer Immunotherapy Program, NCI, NIH, Bethesda, MD, 20892, USA., Highfill SL; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Somerville RP; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Panch SR; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA., Jin P; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA. Pjin@cc.nih.gov., Stroncek DF; Department of Transfusion Medicine, Center for Cellular Engineering, NIH Clinical Center, Bethesda, MD, 20892, USA. DStroncek@cc.nih.gov.

Publikováno v: Journal of translational medicine [J Transl Med] 2022 Nov 08; Vol. 20 (1), pp. 514. Date of Electronic Publication: 2022 Nov 08.

Scaling up and scaling out: Advances and challenges in manufacturing engineered T cell therapies.

Autor: Song HW; National Institutes of Health, Clinical Center, Center for Cellular Engineering, Bethesda, USA., Somerville RP; National Institutes of Health, Clinical Center, Center for Cellular Engineering, Bethesda, USA., Stroncek DF; National Institutes of Health, Clinical Center, Center for Cellular Engineering, Bethesda, USA., Highfill SL; National Institutes of Health, Clinical Center, Center for Cellular Engineering, Bethesda, USA.

Publikováno v: International reviews of immunology [Int Rev Immunol] 2022; Vol. 41 (6), pp. 638-648. Date of Electronic Publication: 2022 Apr 29.