Crosslinking assay to study a specific cargo-coat interaction through a transmembrane receptor in the secretory pathway.

Autor: Javier Manzano-Lopez, Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Ana Maria Perez-Linero, Rafael Lucena, Antonio Cordones-Romero, Sergio Lopez, Auxiliadora Aguilera-Romero, Manuel Muñiz

Publikováno v: PLoS ONE, Vol 17, Iss 2, p e0263617 (2022)

Intracellular trafficking through the secretory organelles depends on transient interactions between cargo proteins and transport machinery. Cytosolic coat protein complexes capture specific luminal cargo proteins for incorporation into transport ves

Externí odkaz: https://doaj.org/article/11b36763662c4d44a1606d0ec1aa449c

Assay for dual cargo sorting into endoplasmic reticulum exit sites imaged by 3D Super-resolution Confocal Live Imaging Microscopy (SCLIM).

Autor: Sofia Rodriguez-Gallardo, Kazuo Kurokawa, Susana Sabido-Bozo, Alejandro Cortes-Gomez, Ana Maria Perez-Linero, Auxiliadora Aguilera-Romero, Sergio Lopez, Miho Waga, Akihiko Nakano, Manuel Muñiz

Publikováno v: PLoS ONE, Vol 16, Iss 10, p e0258111 (2021)

Understanding how in eukaryotic cells thousands of proteins are sorted from each other through the secretory pathway and delivered to their correct destinations is a central issue of cell biology. We have further investigated in yeast how two distinc

Externí odkaz: https://doaj.org/article/559aca166abe4d3295be01873b9fb3a5

The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection

Autor: Susana Sabido-Bozo, Ana Maria Perez-Linero, Javier Manzano-Lopez, Sofia Rodriguez-Gallardo, Auxiliadora Aguilera-Romero, Alejandro Cortes-Gomez, Sergio Lopez, Ralf Erik Wellinger, Manuel Muñiz

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 1, p 423 (2021)

Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites. In yeast,

Externí odkaz: https://doaj.org/article/cbf930ed13794a81b06e72957a29ccde

Endoplasmic Reticulum Export of GPI-Anchored Proteins

Autor: Sergio Lopez, Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Manuel Muñiz

Publikováno v: International Journal of Molecular Sciences, Vol 20, Iss 14, p 3506 (2019)

Protein export from the endoplasmic reticulum (ER) is an essential process in all eukaryotes driven by the cytosolic coat complex COPII, which forms vesicles at ER exit sites for transport of correctly assembled secretory cargo to the Golgi apparatus

Externí odkaz: https://doaj.org/article/042ffc9821644793abbe1d73ccf34a85

The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection

Autor: Javier Manzano-López, Manuel Muñiz, Sergio López, Ralf Erik Wellinger, Ana Maria Perez-Linero, Alejandro Cortes-Gomez, Susana Sabido-Bozo, Sofia Rodriguez-Gallardo, Auxiliadora Aguilera-Romero

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 423, p 423 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 1
idUS. Depósito de Investigación de la Universidad de Sevilla
instname

Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites. In yeast,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec2479e01b4a68af7e1f0d37050e423d
https://www.mdpi.com/1422-0067/22/1/423