Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor

Autor: Schuhmann Wolfgang, Dmytruk Olena V, Smutok Oleh V, Dmytruk Kostyantyn V, Sibirny Andriy A

Publikováno v: BMC Biotechnology, Vol 11, Iss 1, p 58 (2011)

Abstract Background The detection and quantification of uric acid in human physiological fluids is of great importance in the diagnosis and therapy of patients suffering from a range of disorders associated with altered purine metabolism, most notabl

Externí odkaz: https://doaj.org/article/3bf9a9d0c4524c05bacfe615545d965e

Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor

Autor: Schuhmann Wolfgang, Sibirny Volodymyr A, Gayda Galyna Z, Ryabova Olena B, Smutok Oleh V, Dmytruk Kostyantyn V, Gonchar Mykhailo V, Sibirny Andriy A

Publikováno v: BMC Biotechnology, Vol 7, Iss 1, p 33 (2007)

Abstract Background Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming procedure of sample dil

Externí odkaz: https://doaj.org/article/cc496f22ee4b48a1b819816794da3c60

CONSTRUCTION OF AMPEROMETRIC LACCASE-BASED BIOSENSORS USING THE UREASIL AND PHOTOCROSS-LINKED POLYMERS.

Autor: Kavetskyy, Taras S., Kukhazh, Yuliia Y., Zubrytska, Khrystyna V., Khalilov, Rovshan I., Smutok, Oleh V., Demkiv, Olha M., Šauša, Ondrej, Švajdlenková, Helena, Gonchar, Mykhailo V.

Publikováno v: Advances in Biology & Earth Sciences; 2019, Vol. 4 Issue 3, p137-149, 13p

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Flavocytochrome b 2 of the Methylotrophic Yeast Ogataea polymorpha: Construction of Overproducers, Purification, and Bioanalytical Application.

Autor: Smutok OV; Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine. smutok@cellbiol.lviv.ua.; Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine. smutok@cellbiol.lviv.ua.; Clarkson University, Potsdam, NY, USA. smutok@cellbiol.lviv.ua., Dmytruk KV; Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine., Kavetskyy TS; Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine.; The John Paul II Catholic University of Lublin, Lublin, Poland., Sibirny AA; Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine.; University of Rzeszow, Rzeszow, Poland., Gonchar MV; Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2280, pp. 249-260.