Biological plasticity rescues target activity in CRISPR knock outs.

Autor: Smits AH; European Molecular Biology Laboratory, Heidelberg, Germany., Ziebell F; European Molecular Biology Laboratory, Heidelberg, Germany., Joberty G; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany., Zinn N; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany., Mueller WF; European Molecular Biology Laboratory, Heidelberg, Germany., Clauder-Münster S; European Molecular Biology Laboratory, Heidelberg, Germany., Eberhard D; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany., Fälth Savitski M; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany., Grandi P; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany., Jakob P; European Molecular Biology Laboratory, Heidelberg, Germany., Michon AM; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany., Sun H; Department of Genetics and Stanford Genome Technology Center, Stanford University, Stanford, CA, USA., Tessmer K; European Molecular Biology Laboratory, Heidelberg, Germany., Bürckstümmer T; Aelian Biotechnology, Vienna, Austria., Bantscheff M; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany., Steinmetz LM; European Molecular Biology Laboratory, Heidelberg, Germany. lars.steinmetz@embl.de.; Department of Genetics and Stanford Genome Technology Center, Stanford University, Stanford, CA, USA. lars.steinmetz@embl.de., Drewes G; Cellzome, Functional Genomics Research and Development, GlaxoSmithKline, Heidelberg, Germany. gerard.c.drewes@gsk.com., Huber W; European Molecular Biology Laboratory, Heidelberg, Germany. wolfgang.huber@embl.de.

Publikováno v: Nature methods [Nat Methods] 2019 Nov; Vol. 16 (11), pp. 1087-1093. Date of Electronic Publication: 2019 Oct 28.

TRRAP is essential for regulating the accumulation of mutant and wild-type p53 in lymphoma.

Autor: Jethwa A; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany.; Faculty of Biosciences, Heidelberg University, Heidelberg, Germany., Słabicki M; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany., Hüllein J; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany.; Faculty of Biosciences, Heidelberg University, Heidelberg, Germany., Jentzsch M; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany., Dalal V; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany.; Faculty of Biosciences, Heidelberg University, Heidelberg, Germany., Rabe S; Faculty of Biosciences, Heidelberg University, Heidelberg, Germany.; Department of Medicine V, University Hospital Heidelberg, Heidelberg, Germany., Wagner L; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany., Walther T; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany., Klapper W; Hematopathology Section and Lymph Node Registry, Department of Pathology, University Hospital Schleswig-Holstein, Campus Kiel, Christian Albrechts University Kiel, Kiel, Germany., Bohnenberger H; Institute of Pathology, University Medical Center Göttingen, Göttingen, Germany., Rettel M; European Molecular Biology Laboratory, Heidelberg, Germany., Lu J; European Molecular Biology Laboratory, Heidelberg, Germany., Smits AH; European Molecular Biology Laboratory, Heidelberg, Germany., Stein F; European Molecular Biology Laboratory, Heidelberg, Germany., Savitski MM; European Molecular Biology Laboratory, Heidelberg, Germany., Huber W; European Molecular Biology Laboratory, Heidelberg, Germany., Aylon Y; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel; and., Oren M; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel; and., Zenz T; Molecular Therapy in Haematology and Oncology, Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany.; Department of Medicine V, University Hospital Heidelberg, Heidelberg, Germany.; Department of Hematology, University Hospital and University of Zürich, Zürich, Switzerland.

Publikováno v: Blood [Blood] 2018 Jun 21; Vol. 131 (25), pp. 2789-2802. Date of Electronic Publication: 2018 Apr 13.

Phospho-H1 Decorates the Inter-chromatid Axis and Is Evicted along with Shugoshin by SET during Mitosis.

Autor: Krishnan S; Howard Hughes Medical Institute, New York University Langone School of Medicine, New York, NY 10016, USA; Department of Biochemistry and Molecular Pharmacology, New York University Langone School of Medicine, New York, NY 10016, USA., Smits AH; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6525 GA Nijmegen, the Netherlands., Vermeulen M; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6525 GA Nijmegen, the Netherlands., Reinberg D; Howard Hughes Medical Institute, New York University Langone School of Medicine, New York, NY 10016, USA; Department of Biochemistry and Molecular Pharmacology, New York University Langone School of Medicine, New York, NY 10016, USA. Electronic address: danny.reinberg@nyumc.org.

Publikováno v: Molecular cell [Mol Cell] 2017 Aug 17; Vol. 67 (4), pp. 579-593.e6. Date of Electronic Publication: 2017 Aug 03.

Click-MS: Tagless Protein Enrichment Using Bioorthogonal Chemistry for Quantitative Proteomics.

Autor: Smits AH; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen , Nijmegen, The Netherlands., Borrmann A; Department of Bio-organic Chemistry, Faculty of Science, Institute for Molecules and Materials, Radboud University Nijmegen , Nijmegen, The Netherlands., Roosjen M; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen , Nijmegen, The Netherlands.; Department of Bio-organic Chemistry, Faculty of Science, Institute for Molecules and Materials, Radboud University Nijmegen , Nijmegen, The Netherlands., van Hest JC; Department of Bio-organic Chemistry, Faculty of Science, Institute for Molecules and Materials, Radboud University Nijmegen , Nijmegen, The Netherlands., Vermeulen M; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen , Nijmegen, The Netherlands.

Publikováno v: ACS chemical biology [ACS Chem Biol] 2016 Dec 16; Vol. 11 (12), pp. 3245-3250. Date of Electronic Publication: 2016 Oct 17.

The Nucleosome Remodeling and Deacetylase Complex NuRD Is Built from Preformed Catalytically Active Sub-modules.

Autor: Zhang W; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Aubert A; EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France., Gomez de Segura JM; EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France., Karuppasamy M; EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France., Basu S; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Murthy AS; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Diamante A; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Drury TA; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Balmer J; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Cramard J; Wellcome Trust, Medical Research Council Stem Cell Institute, University of Cambridge, Gleeson Building, Tennis Court Road, Cambridge CB2 1QR, United Kingdom., Watson AA; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Lando D; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom., Lee SF; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom., Palayret M; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom., Kloet SL; Department of Molecular Biology, Radboud Institute of Molecular Life Sciences, M850/3.79 Geert Grooteplein Zuid 30, 6525 GA Nijmegen, the Netherlands., Smits AH; Department of Molecular Biology, Radboud Institute of Molecular Life Sciences, M850/3.79 Geert Grooteplein Zuid 30, 6525 GA Nijmegen, the Netherlands., Deery MJ; Cambridge Centre for Proteomics, Cambridge System Biology Centre, Wellcome Trust Stem Cell building, University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QR, United Kingdom., Vermeulen M; Department of Molecular Biology, Radboud Institute of Molecular Life Sciences, M850/3.79 Geert Grooteplein Zuid 30, 6525 GA Nijmegen, the Netherlands., Hendrich B; Wellcome Trust, Medical Research Council Stem Cell Institute, University of Cambridge, Gleeson Building, Tennis Court Road, Cambridge CB2 1QR, United Kingdom., Klenerman D; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom., Schaffitzel C; EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France; The School of Biochemistry, University of Bristol, University Walk, Clifton BS8 1TD, United Kingdom., Berger I; EMBL Grenoble, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France; The School of Biochemistry, University of Bristol, University Walk, Clifton BS8 1TD, United Kingdom., Laue ED; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom.

Publikováno v: Journal of molecular biology [J Mol Biol] 2016 Jul 17; Vol. 428 (14), pp. 2931-42. Date of Electronic Publication: 2016 Apr 23.