Zobrazeno 1 - 10
of 341
pro vyhledávání: '"Smita S. Patel"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract Mitochondrial transcription factor A (TFAM) employs DNA bending to package mitochondrial DNA (mtDNA) into nucleoids and recruit mitochondrial RNA polymerase (POLRMT) at specific promoter sites, light strand promoter (LSP) and heavy strand pr
Externí odkaz:
https://doaj.org/article/d55b655a668c49cd812bca1235890dd7
Autor:
Byeong-Kwon Sohn, Urmimala Basu, Seung-Won Lee, Hayoon Cho, Jiayu Shen, Aishwarya Deshpande, Laura C. Johnson, Kalyan Das, Smita S. Patel, Hajin Kim
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Conformational dynamics during the early stage of transcription is crucial to understanding the regulation of transcription efficiency and fidelity. Here the authors, by single-molecule fluorescence resonance energy transfer approaches, examine the c
Externí odkaz:
https://doaj.org/article/15aa138db4754d9ebf6ec6afbcf7ccc9
Autor:
Sergio E. Martinez, Anupam Singh, Brent De Wijngaert, Shemaila Sultana, Chhaya Dharia, Hans Vanbuel, Jiayu Shen, Daniel Vasilchuk, Smita S. Patel, Kalyan Das
Publikováno v:
STAR Protocols, Vol 2, Iss 2, Pp 100431- (2021)
Summary: In yeast mitochondria, transcription initiation requires assembly of mitochondrial RNA polymerase and transcription initiation factor MTF1 at the DNA promoter initiation site. This protocol describes the purification of the component protein
Externí odkaz:
https://doaj.org/article/1349ced30e2441fba4306d441420bf2c
Autor:
Jie Zheng, Chen Wang, Mi Ra Chang, Swapnil C. Devarkar, Brandon Schweibenz, Gogce C. Crynen, Ruben D. Garcia-Ordonez, Bruce D. Pascal, Scott J. Novick, Smita S. Patel, Joseph Marcotrigiano, Patrick R. Griffin
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
RIG-I is a critical receptor in the induction of innate immune responses, but mutations in RIG-I can be associated with hyperactive signalling and autoimmune disease. Here Zheng et al. apply HDX-MS approaches to reveal dysregulated checkpoints that r
Externí odkaz:
https://doaj.org/article/8317c8b61e4844eb852d67a0ef3d5f02
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
During DNA replication, replicative helicases play an essential role for DNA unwinding to occur. Here the authors find that bacteriophage T7 helicase is also involved in replication re-initiation by interacting with a non-replicating DNAP and increas
Externí odkaz:
https://doaj.org/article/e5b4ac43bbc64c9a8f15790539abd4e6
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
Replicating the genome presents a major challenge to the maintenance of the epigenetic landscape. Here the authors use a minimal experimental system to show how nucleosomes are transferred to double-stranded DNA, with the transfer mediated by a quant
Externí odkaz:
https://doaj.org/article/8dce6f5cb14145d9af93f56261c7fcb6
Autor:
Manjula Pandey, Smita S. Patel
Publikováno v:
Cell Reports, Vol 6, Iss 6, Pp 1129-1138 (2014)
By simultaneously measuring DNA synthesis and dNTP hydrolysis, we show that T7 DNA polymerase and T7 gp4 helicase move in sync during leading-strand synthesis, taking one-nucleotide steps and hydrolyzing one dNTP per base-pair unwound/copied. The coo
Externí odkaz:
https://doaj.org/article/050c1a915b2f4a5d9057d1f9d7ecbcbe
Publikováno v:
Cell Reports, Vol 6, Iss 6, Pp 1037-1045 (2014)
Bacteriophage T7 gp4 serves as a model protein for replicative helicases that couples deoxythymidine triphosphate (dTTP) hydrolysis to directional movement and DNA strand separation. We employed single-molecule fluorescence resonance energy transfer
Externí odkaz:
https://doaj.org/article/d5e71dbc6d404684a08cb06b4dcba64b
Autor:
Brian D. Reed, Michael J. Meyer, Valentin Abramzon, Omer Ad, Pat Adcock, Faisal R. Ahmad, Gün Alppay, James A. Ball, James Beach, Dominique Belhachemi, Anthony Bellofiore, Michael Bellos, Juan Felipe Beltrán, Andrew Betts, Mohammad Wadud Bhuiya, Kristin Blacklock, Robert Boer, David Boisvert, Norman D. Brault, Aaron Buxbaum, Steve Caprio, Changhoon Choi, Thomas D. Christian, Robert Clancy, Joseph Clark, Thomas Connolly, Kathren Fink Croce, Richard Cullen, Mel Davey, Jack Davidson, Mohamed M. Elshenawy, Michael Ferrigno, Daniel Frier, Saketh Gudipati, Stephanie Hamill, Zhaoyu He, Sharath Hosali, Haidong Huang, Le Huang, Ali Kabiri, Gennadiy Kriger, Brittany Lathrop, An Li, Peter Lim, Stephen Liu, Feixiang Luo, Caixia Lv, Xiaoxiao Ma, Evan McCormack, Michele Millham, Roger Nani, Manjula Pandey, John Parillo, Gayatri Patel, Douglas H. Pike, Kyle Preston, Adeline Pichard-Kostuch, Kyle Rearick, Todd Rearick, Marco Ribezzi-Crivellari, Gerard Schmid, Jonathan Schultz, Xinghua Shi, Badri Singh, Nikita Srivastava, Shannon F. Stewman, TR Thurston, T. R. Thurston, Philip Trioli, Jennifer Tullman, Xin Wang, Yen-Chih Wang, Eric A. G. Webster, Zhizhuo Zhang, Jorge Zuniga, Smita S. Patel, Andrew D. Griffiths, Antoine M. van Oijen, Michael McKenna, Matthew D. Dyer, Jonathan M. Rothberg
Publikováno v:
Science. 378:186-192
Studies of the proteome would benefit greatly from methods to directly sequence and digitally quantify proteins and detect posttranslational modifications with single-molecule sensitivity. Here, we demonstrate single-molecule protein sequencing using
Twinkle is the ring-shaped replicative helicase within the human mitochondria with high homology to bacteriophage T7 gp4 helicase-primase. Unlike many orthologs of Twinkle, the N-terminal domain (NTD) of human Twinkle has lost its primase activity th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::757fb42924ec23f8d5337379b368e6bb
https://doi.org/10.1101/2022.11.10.516034
https://doi.org/10.1101/2022.11.10.516034