Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Smita Raghava"'
Autor:
Marc Bailly, Carl Mieczkowski, Veronica Juan, Essam Metwally, Daniela Tomazela, Jeanne Baker, Makiko Uchida, Ester Kofman, Fahimeh Raoufi, Soha Motlagh, Yao Yu, Jihea Park, Smita Raghava, John Welsh, Michael Rauscher, Gopalan Raghunathan, Mark Hsieh, Yi-Ling Chen, Hang Thu Nguyen, Nhung Nguyen, Dan Cipriano, Laurence Fayadat-Dilman
Publikováno v:
mAbs, Vol 12, Iss 1 (2020)
Monoclonal antibodies play an increasingly important role for the development of new drugs across multiple therapy areas. The term ‘developability’ encompasses the feasibility of molecules to successfully progress from discovery to development vi
Externí odkaz:
https://doaj.org/article/adb0a7cc163a47868054a9dee9dd053c
Publikováno v:
PLoS Pathogens, Vol 7, Iss 6, p e1002116 (2011)
Nonenveloped viruses are generally released by the timely lysis of the host cell by a poorly understood process. For the nonenveloped virus SV40, virions assemble in the nucleus and then must be released from the host cell without being encapsulated
Externí odkaz:
https://doaj.org/article/c235e0b53fb84583893cdeaea0ccd46a
Autor:
Dongdong Wang, Jason K. Cheung, Christine Nowak, Yingda Xu, Ning Li, Wei Xu, Bruce Mason, Tony Rossomando, Dingjiang Liu, Diane D Dong, Alain Beck, Joanne Sun, Amit Katiyar, Tao Xiang, Hongcheng Liu, Smita Raghava
Publikováno v:
mAbs. 11:239-264
Increasing attention has been paid to developability assessment with the understanding that thorough evaluation of monoclonal antibody lead candidates at an early stage can avoid delays during late-stage development. The concept of developability is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::403349622c416a603ec56c3e5223e638
https://doi.org/10.1080/19420862.2018.1553476
https://doi.org/10.1080/19420862.2018.1553476
The Viroporin Activity of the Minor Structural Proteins VP2 and VP3 Is Required for SV40 Propagation
Publikováno v:
Journal of Biological Chemistry. 288:2510-2520
For nonenveloped viruses such as Simian Virus 40, the mechanism used to translocate viral components across membranes is poorly understood. Previous results indicated that the minor structural proteins, VP2 and VP3, might act as membrane proteins dur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::764952932f3b68bc68e80783c4acb447
https://doi.org/10.1074/jbc.m112.428425
https://doi.org/10.1074/jbc.m112.428425
Publikováno v:
Journal of Virology. 86:3180-3192
Simian virus 40 (SV40) appears to initiate cell lysis by expressing the late viral protein VP4 at the end of infection to aid in virus dissemination. To investigate the contribution of VP4 to cell lysis, VP4 was expressed in mammalian cells where it
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd5898b1cc6d8fc2a15a809e557b3cca
https://doi.org/10.1128/jvi.07047-11
https://doi.org/10.1128/jvi.07047-11
Publikováno v:
Biochemical Engineering Journal. 42:301-307
Immobilized metal ion affinity chromatography (IMAC) in expanded bed mode is used for purifying recombinant green fluorescent protein (GFP) overexpressed in Escherichia coli. The purification is carried out on two different matrices, i.e. Ni2+ Stream
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::697cb0c667dee9f24ad39efe821cc5b0
https://doi.org/10.1016/j.bej.2008.07.010
https://doi.org/10.1016/j.bej.2008.07.010
Autor:
Kanika Bajaj, Pradeep Singh, Raghavan Varadarajan, Lalima L Madan, Smita Raghava, Mili Das, Munishwar N. Gupta, Balasubramanian Gopal, Bipasha Barua, Sanchari Bhattacharyya
Publikováno v:
Protein Science. 17:1987-1997
Many recombinant eukaryotic proteins tend to form insoluble aggregates called inclusion bodies, especially when expressed in Escherichia coli. We report the first application of the technique of three-phase partitioning (TPP) to obtain correctly refo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ba3a7f0bd5e14ba8f53d174978f11c5
https://doi.org/10.1110/ps.036939.108
https://doi.org/10.1110/ps.036939.108
Autor:
Smita Raghava, Raghavan Varadarajan, Sanchari Bhattacharyya, Samina Aquil, Munishwar N. Gupta
Publikováno v:
Journal of Chromatography A. 1194:90-95
The maltose binding protein (MBP) affinity tag has been extensively used for protein purification. A commercial grade cationic starch could precipitate MBP or an MBP-tagged protein quantitatively by simultaneous addition of 10% (w/v) polyethylene gly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88e7cf50647e42d4b1ad093516e1c12f
https://doi.org/10.1016/j.chroma.2008.04.029
https://doi.org/10.1016/j.chroma.2008.04.029
Publikováno v:
Langmuir. 23:70-75
Alginate, a calcium-sensitive polymer, could carry out simultaneous purification and refolding of 8 M urea/100 mM dithiothreitol (DTT) denatured and thermally denatured alpha-amylase present in a commercial preparation. Activity recoveries of 80 and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b5f49439bf9acd2a2924155a7a1b867
https://doi.org/10.1021/la0616799
https://doi.org/10.1021/la0616799
Publikováno v:
Biochemistry. 52(22)
Nonenveloped viruses are generally released from the cell by the timely lysis of host cell membranes. SV40 has been used as a model virus for the study of the lytic nonenveloped virus life cycle. The expression of SV40 VP4 at later times during infec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28ba3706ec20dc9220c37271e7e22eaa
https://pubmed.ncbi.nlm.nih.gov/23651212
https://pubmed.ncbi.nlm.nih.gov/23651212