Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics.

Autor: Slattery SS; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Giguere DJ; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Stuckless EE; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Shrestha A; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Briere LK; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Galbraith A; Lambton College, 1457 London Rd, Sarnia, ON, N7S 6K4, Canada., Reaume S; Lambton College, 1457 London Rd, Sarnia, ON, N7S 6K4, Canada., Boyko X; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Say HH; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Browne TS; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Frederick MI; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Lant JT; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Heinemann IU; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., O'Donoghue P; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada.; Department of Chemistry, Western University, London, ON, N6A 3K7, Canada., Dsouza L; Pond Technologies Inc., Markham, ON, L3R 9W7, Canada., Martin S; Pond Technologies Inc., Markham, ON, L3R 9W7, Canada., Howard P; Pond Technologies Inc., Markham, ON, L3R 9W7, Canada., Jedeszko C; International Point of Care Inc., 135 The West Mall Unit 9, Toronto, ON, M9C 1C2, Canada., Ali K; International Point of Care Inc., 135 The West Mall Unit 9, Toronto, ON, M9C 1C2, Canada., Styba G; International Point of Care Inc., 135 The West Mall Unit 9, Toronto, ON, M9C 1C2, Canada., Flatley M; Suncor Energy Inc., Sarnia Refinery, 1900 River Road, Sarnia, ON, N7T 7J3, Canada., Karas BJ; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada., Gloor GB; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada. ggloor@uwo.ca., Edgell DR; Department of Biochemistry, Schlich School of Medicine and Dentistry, Western University, London, ON, N6A 5C1, Canada. dedgell@uwo.ca.

Publikováno v: Scientific reports [Sci Rep] 2022 Apr 29; Vol. 12 (1), pp. 7010. Date of Electronic Publication: 2022 Apr 29.

Delivery of the Cas9 or TevCas9 system into Phaeodactylum tricornutum via conjugation of plasmids from a bacterial donor.

Autor: Wang H; Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London, ON, Canada., Slattery SS; Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London, ON, Canada., Karas BJ; Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London, ON, Canada.; Designer Microbes Inc., 700 Collip Circle, London ON N6G 4X8, Canada., Edgell DR; Department of Biochemistry, Schulich School of Medicine & Dentistry, Western University, London, ON, Canada.

Publikováno v: Bio-protocol [Bio Protoc] 2018 Aug 20; Vol. 8 (16), pp. e2974. Date of Electronic Publication: 2018 Aug 20 (Print Publication: 2018).