Screening Clones for Monoclonal Antibody Production Using Droplet Microfluidics Interfaced to Electrospray Ionization Mass Spectrometry.

Autor: D'Amico CI; Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, United States., Robbins G; Department of Chemistry, University of Michigan, Ann Arbor Michigan 48109, United States., Po I; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Fang Z; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Slaney TR; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Tremml G; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Tao L; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Ruotolo BT; Department of Chemistry, University of Michigan, Ann Arbor Michigan 48109, United States., Kennedy RT; Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, United States.; Department of Chemistry, University of Michigan, Ann Arbor Michigan 48109, United States.

Publikováno v: Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2023 Jun 07; Vol. 34 (6), pp. 1117-1124. Date of Electronic Publication: 2023 May 16.

Enzymatic removal of sialic acid enables iCIEF stability monitoring of charge variants of a highly sialylated bispecific antibody.

Autor: Peters BH; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Bautista J; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Slaney TR; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Guo H; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Huang RY; Pharmaceutical Candidate Optimization, Bristol Myers Squibb, Lawrence Township, New Jersey, USA., Krause ME; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Zeng M; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Cheng J; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Chen Z; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA.

Publikováno v: Electrophoresis [Electrophoresis] 2022 May; Vol. 43 (9-10), pp. 1059-1067. Date of Electronic Publication: 2022 Apr 27.

Unique Impacts of Methionine Oxidation, Tryptophan Oxidation, and Asparagine Deamidation on Antibody Stability and Aggregation.

Autor: Alam ME; Isermann Department of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180., Slaney TR; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Wu L; Department of Chemical Engineering, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109., Das TK; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Kar S; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Barnett GV; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Leone A; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Tessier PM; Isermann Department of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180; Department of Chemical Engineering, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109; Department of Pharmaceutical Sciences, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109; Department of Biomedical Engineering, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109. Electronic address: ptessier@umich.edu.

Publikováno v: Journal of pharmaceutical sciences [J Pharm Sci] 2020 Jan; Vol. 109 (1), pp. 656-669. Date of Electronic Publication: 2019 Oct 31.

Codon-Directed Determination of the Biological Causes of Sequence Variants in Therapeutic Proteins.

Autor: Jiang T; Department of Chemistry, The University of Michigan , 930 North University Avenue, Ann Arbor, Michigan 48109, United States., Song H; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., Slaney TR; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., Wu W; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., Langsdorf E; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., Gupta G; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., Ludwig R; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., Tao L; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., McVey D; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States., Das TK; Analytical, Cell Line and Process Development, Bristol-Myers Squibb Company , 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States.

Publikováno v: Analytical chemistry [Anal Chem] 2017 Dec 05; Vol. 89 (23), pp. 12749-12755. Date of Electronic Publication: 2017 Nov 14.

Chemical gradients within brain extracellular space measured using low flow push-pull perfusion sampling in vivo.

Autor: Slaney TR; Department of Chemistry, University of Michigan, 930 N. University Ave. Ann Arbor, MI 48109, USA., Mabrouk OS, Porter-Stransky KA, Aragona BJ, Kennedy RT

Publikováno v: ACS chemical neuroscience [ACS Chem Neurosci] 2013 Feb 20; Vol. 4 (2), pp. 321-9. Date of Electronic Publication: 2012 Nov 16.