Screening Clones for Monoclonal Antibody Production Using Droplet Microfluidics Interfaced to Electrospray Ionization Mass Spectrometry.

Autor: D'Amico CI; Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, United States., Robbins G; Department of Chemistry, University of Michigan, Ann Arbor Michigan 48109, United States., Po I; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Fang Z; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Slaney TR; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Tremml G; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Tao L; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey 08901, United States., Ruotolo BT; Department of Chemistry, University of Michigan, Ann Arbor Michigan 48109, United States., Kennedy RT; Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, United States.; Department of Chemistry, University of Michigan, Ann Arbor Michigan 48109, United States.

Publikováno v: Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2023 Jun 07; Vol. 34 (6), pp. 1117-1124. Date of Electronic Publication: 2023 May 16.

Enzymatic removal of sialic acid enables iCIEF stability monitoring of charge variants of a highly sialylated bispecific antibody.

Autor: Peters BH; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Bautista J; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Slaney TR; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Guo H; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Huang RY; Pharmaceutical Candidate Optimization, Bristol Myers Squibb, Lawrence Township, New Jersey, USA., Krause ME; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Zeng M; Biologics Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Cheng J; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA., Chen Z; Drug Product Development, Bristol Myers Squibb, New Brunswick, New Jersey, USA.

Publikováno v: Electrophoresis [Electrophoresis] 2022 May; Vol. 43 (9-10), pp. 1059-1067. Date of Electronic Publication: 2022 Apr 27.

Unique Impacts of Methionine Oxidation, Tryptophan Oxidation, and Asparagine Deamidation on Antibody Stability and Aggregation.

Autor: Alam ME; Isermann Department of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180., Slaney TR; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Wu L; Department of Chemical Engineering, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109., Das TK; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Kar S; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Barnett GV; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Leone A; Biologics Development, Bristol-Myers Squibb, Pennington, New Jersey 08534., Tessier PM; Isermann Department of Chemical & Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180; Department of Chemical Engineering, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109; Department of Pharmaceutical Sciences, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109; Department of Biomedical Engineering, Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109. Electronic address: ptessier@umich.edu.

Publikováno v: Journal of pharmaceutical sciences [J Pharm Sci] 2020 Jan; Vol. 109 (1), pp. 656-669. Date of Electronic Publication: 2019 Oct 31.

Stability of Protein Pharmaceuticals: Recent Advances.

Autor: Manning MC; Legacy BioDesign LLC, Johnstown, CO, USA. manning@legacybiodesign.com.; Department of Chemistry, Colorado State University, Fort Collins, CO, USA. manning@legacybiodesign.com., Holcomb RE; Legacy BioDesign LLC, Johnstown, CO, USA.; Department of Chemistry, Colorado State University, Fort Collins, CO, USA., Payne RW; Legacy BioDesign LLC, Johnstown, CO, USA.; Department of Chemistry, Colorado State University, Fort Collins, CO, USA., Stillahn JM; Legacy BioDesign LLC, Johnstown, CO, USA.; Department of Chemistry, Colorado State University, Fort Collins, CO, USA., Connolly BD; Spyre Therapeutics, Inc., Waltham, MA, USA., Katayama DS; Samsung Biologics, 201-2, Songdo-Dong, Yeonsu-Gu, Incheon, Korea., Liu H; Scholar Rock, Inc., Cambridge, MA, USA., Matsuura JE; Jim Matsuura Pharma Consulting LLC, Lafayette, CO, USA., Murphy BM; Just-Evotec, Seattle, WA, USA., Henry CS; Department of Chemistry, Colorado State University, Fort Collins, CO, USA., Crommelin DJA; Utrecht University, Utrecht, Netherlands.

Publikováno v: Pharmaceutical research [Pharm Res] 2024 Jul; Vol. 41 (7), pp. 1301-1367. Date of Electronic Publication: 2024 Jun 27.

LC-MS Characterization and Stability Assessment Elucidate Correlation Between Charge Variant Composition and Degradation of Monoclonal Antibody Therapeutics.

Autor: Malani H; Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India., Shrivastava A; Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India., Nupur N; Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India., Rathore AS; Department of Chemical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India. asrathore@biotechcmz.com.

Publikováno v: The AAPS journal [AAPS J] 2024 Apr 03; Vol. 26 (3), pp. 42. Date of Electronic Publication: 2024 Apr 03.