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GATA1-deficient human pluripotent stem cells generate neutrophils with improved antifungal immunity that is mediated by the integrin CD18.

Autor: Wagner AS; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA., Smith FM; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA., Bennin DA; Department of Pediatrics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA., Votava JA; Morgridge Institute for Research, Madison, WI, USA., Datta R; Morgridge Institute for Research, Madison, WI, USA., Giese MA; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA., Zhao W; Morgridge Institute for Research, Madison, WI, USA., Skala MC; Morgridge Institute for Research, Madison, WI, USA., Fan J; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.; Morgridge Institute for Research, Madison, WI, USA., Keller NP; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.; Department of Plant Pathology, University of Wisconsin-Madison, WI, USA., Huttenlocher A; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.; Department of Pediatrics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Oct 11. Date of Electronic Publication: 2024 Oct 11.

Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.

Autor: Juang TD; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu., Riendeau J; Morgridge Institute for Research, Madison, WI, USA. skerr2@wisc.edu., Geiger PG; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA., Datta R; Morgridge Institute for Research, Madison, WI, USA. skerr2@wisc.edu., Lares M; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA., Yada RC; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA., Singh AM; Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, WI, USA., Seroogy CM; Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, WI, USA., Gern JE; Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, WI, USA., Skala MC; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu.; Morgridge Institute for Research, Madison, WI, USA. skerr2@wisc.edu.; University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI, USA., Beebe DJ; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu.; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA.; University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI, USA., Kerr SC; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. djbeebe@wisc.edu.; University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI, USA.

Publikováno v: Lab on a chip [Lab Chip] 2024 Aug 20; Vol. 24 (17), pp. 4198-4210. Date of Electronic Publication: 2024 Aug 20.

Naive primary neutrophils play a dual role in the tumor microenvironment.

Autor: Babatunde KA; Department of Pathology & Laboratory Medicine, University of Wisconsin, Madison, WI 53705, USA., Datta R; Morgridge Institute for Research, Madison, WI 53715, USA., Hendrikse NW; Department of Pathology & Laboratory Medicine, University of Wisconsin, Madison, WI 53705, USA., Ayuso JM; Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53715, USA.; Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA.; Department of Dermatology, University of Wisconsin, Madison, WI 53705, USA., Huttenlocher A; Departments of Pediatrics and Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53792, USA., Skala MC; Morgridge Institute for Research, Madison, WI 53715, USA.; Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53715, USA.; Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA., Beebe DJ; Department of Pathology & Laboratory Medicine, University of Wisconsin, Madison, WI 53705, USA.; Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53715, USA.; Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA., Kerr SC; Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53715, USA.; Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA.

Publikováno v: IScience [iScience] 2024 Aug 02; Vol. 27 (9), pp. 110632. Date of Electronic Publication: 2024 Aug 02 (Print Publication: 2024).

Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems.

Autor: Datta R, Miskolci V, Gallego-López GM, Britt E, Gillette A, Kralovec A, Giese MA, Qian T, Fan J, Huttenlocher A, Skala MC

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jul 29. Date of Electronic Publication: 2024 Jul 29.