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Rapid quantification of microRNA-375 through one-pot primer-generating rolling circle amplification

Autor: Lucas D. Smith, Siva Nalla, Chia-Wei Kuo, Manish Kohli, Andrew M. Smith

Publikováno v: The Analyst. 147:2936-2941

A recent surge of interest in microRNA has been driven by its discovery as a circulating biomarker of disease, with many diagnostic test platforms currently under development. Alternatives to widely used microRNA quantification methods such as quanti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdc3b3e99b2876142fd62f29fd0b4f1e
https://doi.org/10.1039/d2an00263a

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Abstract 4317: Comparison of Single-Molecule Flow (SiM-Flow) and qRT-PCR for detection of plasma miR-375 and miR-1290 in metastatic castrate-resistant prostate cancer (mCRPC)

Autor: Manish Kohli, Siva Nalla, Chia-Wei Kuo, Claire Hanson, Matt Larsen, Anna Neibling, Jennifer Lloyd, Julia Batten, Neeraj Agarwal, Umang Swami, Benjamin Maughan, Sumati Gupta, Rebecca L. Smith, Andrew Smith

Publikováno v: Cancer Research. 83:4317-4317

Background: Plasma microRNA (miR)-375 and miR-1290 levels have been associated with poor survival and resistance to docetaxel chemotherapy. Detection of plasma miRs is typically performed using qRT-PCR, which is time-intensive and laborious. We devel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::424b219a702e7eb4b492ba3fedd1f486
https://doi.org/10.1158/1538-7445.am2023-4317

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