Zobrazeno 1 - 10
of 825
pro vyhledávání: '"Single-molecule localization microscopy"'
Autor:
Nan Jiang, Andras Saftics, Eugenia Romano, Ima Ghaeli, Cristal Resto, Vanessa Robles, Saumya Das, Kendall Van Keuren-Jensen, Victoria L. Seewaldt, Tijana Jovanovic-Talisman
Publikováno v:
Journal of Nanobiotechnology, Vol 22, Iss 1, Pp 1-16 (2024)
Abstract Background Patients with HER2-positive breast cancer can significantly benefit from HER2-directed therapy – such as the monoclonal antibody trastuzumab. However, some patients can develop therapy resistance or change HER2 status. Thus, we
Externí odkaz:
https://doaj.org/article/dfd432c8e2da4fa18fab1236c2c53ce7
Autor:
Jia Zhe, Zhou Lingxiao, Li Haoyu, Ni Jielei, Chen Danni, Guo Dongfei, Cao Bo, Liu Gang, Liang Guotao, Zhou Qianwen, Yuan Xiaocong, Ni Yanxiang
Publikováno v:
Nanophotonics, Vol 13, Iss 19, Pp 3647-3661 (2024)
Precisely pinpointing the positions of emitters within the diffraction limit is crucial for quantitative analysis or molecular mechanism investigation in biomedical research but has remained challenging unless exploiting single molecule localization
Externí odkaz:
https://doaj.org/article/dfa07af59e1b497899b916ef031d941e
Autor:
Jeong Uidon, Go Ga-eun, Jeong Dokyung, Lee Dongmin, Kim Min Jeong, Kang Minjae, Kim Namyoon, Jung Jaehwang, Kim Wookrae, Lee Myungjun, Kim Doory
Publikováno v:
Nanophotonics, Vol 13, Iss 2, Pp 195-207 (2024)
The recent advances in super-resolution fluorescence microscopy, including single-molecule localization microscopy (SMLM), has enabled the study of previously inaccessible details, such as the organization of proteins within cellular compartments and
Externí odkaz:
https://doaj.org/article/38b4012063e04d97a95dc9de81e18359
Autor:
Fabian Hauser, Christoph Naderer, Eleni Priglinger, Anja Peterbauer, Michael B. Fischer, Heinz Redl, Jaroslaw Jacak
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
A biotechnological platform consisting of two-color 3D super-resolution readout and a microfluidic system was developed to investigate platelet interaction with a layer of perfused endothelial cells under flow conditions. Platelet activation has been
Externí odkaz:
https://doaj.org/article/7668df213e744a338ea299e6f6222b0f
Publikováno v:
Open Biology, Vol 14, Iss 2 (2024)
In this work, we have developed an expansion microscopy (ExM) protocol that combines ExM with photoactivated localization microscopy (ExPALM) for yeast cell imaging, and report a robust protocol for single-molecule and expansion microscopy of fission
Externí odkaz:
https://doaj.org/article/ac58b05875ed4ef49d059391a6babd71
Autor:
Øystein Øvrebø, Miina Ojansivu, Kimmo Kartasalo, Hanna M. G. Barriga, Petter Ranefall, Margaret N. Holme, Molly M. Stevens
Publikováno v:
BMC Bioinformatics, Vol 24, Iss 1, Pp 1-18 (2023)
Abstract Background Stochastic optical reconstruction microscopy (STORM), a super-resolution microscopy technique based on single-molecule localizations, has become popular to characterize sub-diffraction limit targets. However, due to lengthy image
Externí odkaz:
https://doaj.org/article/22d04680a034445f873b0e70e917aec8
Publikováno v:
Journal of Innovative Optical Health Sciences, Vol 16, Iss 06 (2023)
Quantitative data analysis in single-molecule localization microscopy (SMLM) is crucial for studying cellular functions at the biomolecular level. In the past decade, several quantitative methods were developed for analyzing SMLM data; however, imagi
Externí odkaz:
https://doaj.org/article/82733f82e1ff4fc5b11e2157ba3ef8b8
Publikováno v:
Nano Convergence, Vol 10, Iss 1, Pp 1-14 (2023)
Abstract Single-molecule localization microscopy (SMLM) breaks the optical diffraction limit by numerically localizing sparse fluorescence emitters to achieve super-resolution imaging. Spectroscopic SMLM or sSMLM further allows simultaneous spectrosc
Externí odkaz:
https://doaj.org/article/b83aca69419c4aaa9cde6f7fd0087544
Autor:
Jonas Weidner, Charlotte Neitzel, Martin Gote, Jeanette Deck, Kim Küntzelmann, Götz Pilarczyk, Martin Falk, Michael Hausmann
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 21, Iss , Pp 2018-2034 (2023)
The cell as a system of many components, governed by the laws of physics and chemistry drives molecular functions having an impact on the spatial organization of these systems and vice versa. Since the relationship between structure and function is a
Externí odkaz:
https://doaj.org/article/e7d1bad348bb4894b790baf70549be24
Autor:
Yoonsuk Hyun, Doory Kim
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 21, Iss , Pp 879-888 (2023)
With the development of super-resolution imaging techniques, it is crucial to understand protein structure at the nanoscale in terms of clustering and organization in a cell. However, cluster analysis from single-molecule localization microscopy (SML
Externí odkaz:
https://doaj.org/article/1a2c5859bef541f180d95a8dc5dd8aea