Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Sineli R. Silveira"'
Catalases and peroxidases histochemical detection; techniques suitable to discriminate these enzymes
Autor:
Walter A. Hadler, Sineli R. Silveira
Publikováno v:
Acta histochemica. 63(1)
Summary By using the benzidine reaction, on filter paper strips loaded with catalases, peroxidases, porphyrins, haemic iron compounds and iron salts, it was possible to establish 2 histochemical techniques able to detect and discriminate catalases an
Publikováno v:
Acta histochemica. 63(2)
Summary An almost specific and highly sensitive technique to detect histochemically choline-containing lipids is proposed. This technique is based on the effect of Hg ++ and phosphomolybdic ion on the choline, that yields insoluble complexes able to
Autor:
Sineli R. Silveira, Walter A. Hadler
Publikováno v:
Acta histochemica. 76(2)
Summary A suitable histochemical method able to discriminate vitamins D and 7-dehydrocholesterol from cholesterol and its esters is proposed taking into account the alkaline permanganate-toluidine blue reaction, the alkaline permanganate-Schiff react
Autor:
Sineli R. Silveira, Walter A. Hadler
Publikováno v:
Acta histochemica. 74(1)
The peracetic acid-toluidine blue and the peracetic acid-Schiff reactions as well as the failure of HgCl2-formalin fixative for inhibiting these reactions appears as a very useful method for free cholesterol histochemical detection and to discriminat
Autor:
Walter A. Hadler, Sineli R. Silveira
Publikováno v:
Acta histochemica. 68(1)
The epidermis upper layer reacts positively to the performic acid-toluidine blue and the performic acid-Schiff; such reactions were inhibited by a previous formalin-HgCl2 fixation. The substance accounted for that reactivity could be extracted from s
Autor:
Sineli R. Silveira, Walter A. Hadler
Publikováno v:
Acta Histochemica. 61:223-INS2
Summary As an attempt to test the specificity of an histochemical technique proposed to detect catalases, an investigation was carried out by immunochemical techniques. Purified catalases were used after analysed immunochemically by double immune dif
Autor:
Sineli R. Silveira, Walter A. Hadler
Publikováno v:
Acta Histochemica. 76:225-IN3
Using a suitable histochemical method vitamins D and 7-dehydrocholesterol could be shown into the epidermis of several mammal species. As the histochemical method used is able to discriminate vitamins D and 7-dehydrocholesterol from cholesterol and i
Autor:
Sineli R. Silveira, Walter A. Hadler
Publikováno v:
Acta Histochemica. 77:11-IN2
Summary The vitamin D transepidermis absorption was studied by means of a histochemical technique suitable to detect this vitamin and to discriminate it from cholesterol and its esters. Such technique shows vitamin D inside the mast cell granules. As
Autor:
Sineli R. Silveira, Walter A. Hadler
Publikováno v:
Acta Histochemica. 68:1-IN1
A technique to detect tocopherol histochemically was proposed in basis on the following schedule: 1. toluidine blue and Schiff reagent negativity before a suitable oxidation; 2. performic acid-toluidine blue and performic acid-Schiff reagent positivi