Discovery of an Active RAG Transposon Illuminates the Origins of V(D)J Recombination

Autor: Ya Zhang, David G. Schatz, Anlong Xu, Wenjuan Yu, Yuhang Zhang, Xiaolong Liu, Yann Le Petillon, Shengfeng Huang, Helen A. Beilinson, Xin Tao, Shaochun Yuan, Hector Escriva, Peiyi Li, Pierre Pontarotti, Shangwu Chen

Publikováno v: Cell
Cell, Elsevier, 2016, 166 (1), pp.102-114. ⟨10.1016/j.cell.2016.05.032⟩
Cell, 2016, 166 (1), pp.102-114. ⟨10.1016/j.cell.2016.05.032⟩

International audience; Co-option of RAG1 and RAG2 for antigen receptor gene assembly by V(D)J recombination was a crucial event in the evolution of jawed vertebrate adaptive immunity. RAG1/2 are proposed to have arisen from a transposable element, b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::414b3d9472642fa700cd8c3e21941a50
https://doi.org/10.1016/j.cell.2016.05.032

Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon

Autor: Christian Frøkjær-Jensen, Erik M. Jorgensen, Stephane Flibotte, Mihail Sarov, Matthew L. LaBella, Jon Taylor, M. Wayne Davis, Andrei Pozniakovsky, Donald G. Moerman

Publikováno v: Nature Methods. 11:529-534

We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bd5e6ab96d76d5f5d523720ec39b62b5
https://doi.org/10.1038/nmeth.2889

A bend, flip and trap mechanism for transposon integration

Autor: Elizabeth R. Morris, Heather Grey, Julia M. Richardson, Anita C. Jones, Grant McKenzie

Publikováno v: eLife
eLife, Vol 5 (2016)
Morris, E, Grey, H, McKenzie, G, Jones, A & Richardson, J 2016, ' A bend, flip and trap mechanism for transposon integration ', eLIFE, vol. 5, e15537 . https://doi.org/10.7554/eLife.15537

Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b5acf154aff51b451802b20d022c629
http://europepmc.org/articles/PMC5481204

Transposition of Mboumar-9: Identification of a New Naturally Active mariner-Family Transposon

Autor: Ronald Chalmers, Martín Muñoz-López, Julien Bischerour, Pedro Lorite, Azeem Siddique, Teresa Palomeque

Publikováno v: Journal of Molecular Biology

Although mariner transposons are widespread in animal genomes, the vast majority harbor multiple inactivating mutations and only two naturally occurring elements are known to be active. Previously, we discovered a mariner-family transposon, Mboumar,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fb5c380875348f384b318567a640edb
https://doi.org/10.1016/j.jmb.2008.07.044

Mechanism of IS200/IS605 Family DNA Transposases: Activation and Transposon-Directed Target Site Selection

Autor: Michael Chandler, Alison B. Hickman, Fred Dyda, Bao Ton-Hoang, Orsolya Barabas, Donald R. Ronning, Catherine Guynet

Publikováno v: Cell
Cell, Elsevier, 2008, pp.208-220

SummaryThe smallest known DNA transposases are those from the IS200/IS605 family. Here we show how the interplay of protein and DNA activates TnpA, the Helicobacter pylori IS608 transposase, for catalysis. First, transposon end binding causes a confo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e1516661ba1bffd954b4bc6ca34db01