Výsledky vyhledávání - "Simone S, Azzolini"

Validation of a Phage Display Method for Protease Inhibitor Selection Using SFTI and HiTI Synthetic Hybrid Peptides

Autor: Antonio Miranda, Rogerio Amino, Aparecida S. Tanaka, Renato de Marco, Diogo V. Lovato, Ricardo J.S. Torquato, Simone S. Azzolini

Publikováno v: ResearcherID

A recombinant Haematobia irritans irritans trypsin inhibitor (HiTI - Mw 7030 kDa) phagemid library was constructed and displayed functionally on the tip of the filamentous M13 phage. A combinatorial library of 7.2 x 106 mutants was created with HiTI

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fea1cd51e821cd881e5b4d0b433acaf
https://doi.org/10.2174/138620710792927394

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Validation of phage display method for protease inhibitor selection using synthetic hybrid peptides

Autor: Renato, de Marco, Simone S, Azzolini, Diogo V, Lovato, Ricardo J S, Torquato, Rogerio, Amino, Antonio, de Miranda, Aparecida S, Tanaka

Publikováno v: Combinatorial chemistryhigh throughput screening. 13(9)

A recombinant Haematobia irritans irritans trypsin inhibitor (HiTI - Mw 7030 kDa)) phagemid library was constructed and displayed functionally on the tip of the filamentous M13 phage. A combinatorial library of 7.2 x 10(6) mutants was created with Hi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::02dcc7ec1a7018db016ce4e332af0898
https://pubmed.ncbi.nlm.nih.gov/20615196

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The role of HiTI, a serine protease inhibitor from Haematobia irritans irritans (Diptera: Muscidae) in the control of fly and bacterial proteases

Autor: Sergio D. Sasaki, Aparecida S. Tanaka, Maria A. Juliano, Simone S. Azzolini, Ricardo J.S. Torquato, I. T. N. Campos

Publikováno v: Experimental parasitology. 111(1)

Blood-sucking arthropods are vectors responsible for the transmission of several pathogens and parasites to vertebrate animals. The horn fly Haematobia irritans irritans (Diptera: Muscidae) and the tick Boophilus microplus are important hematophagous

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9285d5eec1c153a93abeac0a9872d616
https://pubmed.ncbi.nlm.nih.gov/16054488

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Evaluation of phage display system and leech-derived tryptase inhibitor as a tool for understanding the serine proteinase specificities

Autor: Hans Fritz, Adriana F. Souza, Aparecida S. Tanaka, Simone S. Azzolini, Melissa Andreia de Moraes Silva, Claudio A. M. Sampaio, I. T. N. Campos

Publikováno v: Archives of biochemistry and biophysics. 425(1)

A small combinatorial library of LDTI mutants (5.2 x 10(4)) restricted to the P1-P4' positions of the reactive site was displayed on the pCANTAB 5E phagemid, and LDTI fusion phages were produced and selected for potent neutrophil elastase and plasmin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0446d963fdd3eedbce2627f6171233e
https://pubmed.ncbi.nlm.nih.gov/15081897

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Boophilus microplus tick larvae, a rich source of Kunitz type serine proteinase inhibitors

Autor: Izaura Y. Hirata, Renato Andreotti, Aparecida S. Tanaka, Simone S. Azzolini, Sergio D. Sasaki

Publikováno v: Biochimie. 86(9-10)

Serine proteinase inhibitors from Boophilus microplus tick larvae (BmTIs) were purified by affinity chromatography on a trypsin-Sepharose column. BmTIs presented molecular weight between M(r) 6200 and 18,400 and inhibitory activity for trypsin, HuPK

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a269b4c0074583a790a729961a0519c6
https://pubmed.ncbi.nlm.nih.gov/15556274

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Rhipicephalus sanguineus trypsin inhibitors present in the tick larvae: isolation, characterization, and partial primary structure determination

Autor: Ricardo J.S. Torquato, Aparecida S. Tanaka, Simone S. Azzolini, Renato Andreotti, Sergio D. Sasaki, Eliane Andreotti

Publikováno v: Archives of biochemistry and biophysics. 417(2)

Blood sucking animals are a rich source of proteinase inhibitors mainly those that interfere in their host hemostatic systems. The tick Rhipicephalus sanguineus is an ectoparasite of dogs and other animals. The aims of this work were the purification

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4b6810480c184d5400928702c08070b
https://pubmed.ncbi.nlm.nih.gov/12941299

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Purification, characterization, and cloning of a serine proteinase inhibitor from the ectoparasite Haematobia irritans irritans (Diptera: Muscidae)

Autor: Aparecida S. Tanaka, Simone S. Azzolini, Joyce M.C. Santos, Ricardo J.S. Torquato, Izaura Y. Hirata, Renato Andreotti, Adriana F. Souza

Publikováno v: Experimental parasitology. 106(3-4)

The fly Haematobia irritans irritans is one of the most important ectoparasites in cattle production, due to its ability to suck large amounts of blood. This report describes the purification and characterization of a serine proteinase inhibitor (HiT

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28af3677bf17ace2113bb6e32360864b
https://pubmed.ncbi.nlm.nih.gov/15172217

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