Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Simon Wadle"'
Autor:
Lisa Becherer, Jacob Friedrich Hess, Sieghard Frischmann, Mohammed Bakheit, Hans Nitschko, Silvina Stinco, Friedrich Zitz, Hannes Hofer, Giampiero Porro, Florian Hausladen, Karl Stock, Dominik Drossart, Holger Wurm, Hanna Kuhn, Dominik Huber, Tobias Hutzenlaub, Nils Paust, Mark Keller, Oliver Strohmeier, Simon Wadle, Nadine Borst, Roland Zengerle, Felix von Stetten
Publikováno v:
Micromachines, Vol 12, Iss 2, p 159 (2021)
This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD
Externí odkaz:
https://doaj.org/article/4a5b12acd76d4d2a9e78791e90dfe337
Autor:
Simon Wadle, Michael Lehnert, Friedrich Schuler, René Köppel, Annerose Serr, Roland Zengerle, Felix von Stetten
Publikováno v:
BioTechniques, Vol 61, Iss 3, Pp 123-128 (2016)
Mediator probe (MP) PCR is a real-time PCR approach that uses standardized universal fluorogenic reporter oligonucleotides (UR) in conjunction with label-free sequence-specific probes. To enable multiplex real-time MP PCR, we designed a set of five o
Externí odkaz:
https://doaj.org/article/f4d9af57eaa7470fabf45325be843c3f
Autor:
Markus Rombach, Dominique Kosse, Bernd Faltin, Simon Wadle, Günter Roth, Roland Zengerle, Felix von Stetten
Publikováno v:
BioTechniques, Vol 57, Iss 3, Pp 151-155 (2014)
Externí odkaz:
https://doaj.org/article/5cdbbbccd25649d5886694d7e7c041c1
Autor:
Roland Zengerle, Michael Jehle, Mara Specht, Florian Hausladen, Simon Wadle, Silvia Calabrese, Ulrike Goetz, Nadine Borst, Anna M. Sobieraj, Holger Wurm, Annerose Serr, Martin J. Loessner, Anja Gerhardts, Philip Koch, Raimund Rother, Karl Stock, Mathias Schmelcher, Felix von Stetten, Martin Schulz, Dominik Drossart, Nils Paust, Jia Li, Philipp Tepper, Martin Meyer, Marina Handel, Georg Haecker, Fritz Eichenseher
Publikováno v:
Lab on a Chip. 20:2549-2561
We present an automated point-of-care testing (POCT) system for rapid detection of species- and resistance markers in methicillin-resistant Staphylococcus aureus (MRSA) at the level of single cells, directly from nasal swab samples. Our novel system
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::982bac2df81f51cd64f35b6b7276f8f3
https://doi.org/10.1039/d0lc00294a
https://doi.org/10.1039/d0lc00294a
Autor:
Ana Belén Rodríguez-Martínez, Simon Wadle, Lisa Becherer, Jia Li, Mara Specht, Martin Trotter, Friedrich Schuler, Martin Schulz, Roland Zengerle, Nadine Borst, Felix von Stetten, Nils Paust
Publikováno v:
LaboratoriumsMedizin. 41:245-249
The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Diffe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6bb7500ea43dd754e7e14dc4f7cad3eb
https://doi.org/10.1515/labmed-2017-0098
https://doi.org/10.1515/labmed-2017-0098
Autor:
Friedrich Zitz, Hans Nitschko, Mohammed A. Bakheit, Karl Stock, Florian Hausladen, Dominik Drossart, Nils Paust, Giampiero Porro, Oliver Strohmeier, Roland Zengerle, Felix von Stetten, Nadine Borst, Hannes Hofer, Silvina Stinco, Mark Keller, Holger Wurm, Lisa Becherer, Dominik Huber, Simon Wadle, Sieghard Frischmann, Hanna Kuhn, Jacob Friedrich Hess, Tobias Hutzenlaub
Publikováno v:
Micromachines
Volume 12
Issue 2
Micromachines, Vol 12, Iss 159, p 159 (2021)
Volume 12
Issue 2
Micromachines, Vol 12, Iss 159, p 159 (2021)
This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ccf0ec184a557b3045131689c5c6b80
https://doi.org/10.3390/mi12020159
https://doi.org/10.3390/mi12020159
Autor:
Roland Zengerle, Clara Siber, Nils Paust, Simon Wadle, Felix von Stetten, Friedrich Schuler, Sebastian Hin
Publikováno v:
Analytical Methods. 8:2750-2755
Digital nucleic acid amplification methods are a growing research field that allows for absolute quantification of DNA making the need of standard curves redundant. However, most of the existing digital amplification systems require specialized labor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6658e54d439a880ebff89505d3dd759b
https://doi.org/10.1039/c6ay00600k
https://doi.org/10.1039/c6ay00600k
Publikováno v:
Biomolecular Detection and Quantification
Biomolecular Detection and Quantification, Vol 7, Iss C, Pp 1-8 (2016)
Biomolecular Detection and Quantification, Vol 7, Iss C, Pp 1-8 (2016)
Graphical abstract
Highlights • Design of experiments (DOE) is an appropriate approach for probe sequence optimization in real-time PCR. • Nine MP designs led to maximum information for the 3 input factors. • Using DOE the maximum number o
Highlights • Design of experiments (DOE) is an appropriate approach for probe sequence optimization in real-time PCR. • Nine MP designs led to maximum information for the 3 input factors. • Using DOE the maximum number o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca554001d0a01039fba170f041f078ca
http://europepmc.org/articles/PMC4827641
http://europepmc.org/articles/PMC4827641
Autor:
Simon Wadle, C. Nuese, F. von Stetten, Oliver Strohmeier, Mark Keller, L. Dreesen, Nils Paust, Roland Zengerle
Publikováno v:
RSC Advances. 5:89603-89611
We introduce microfluidic automation of geometrically multiplexed real-time PCR to off-the-shelf Rotor-Gene Q thermocyclers (RGQ, QIAGEN GmbH, Hilden, Germany). For centrifugal fluid control the RGQ provides low and constant rotation of 400 rpm, only
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9f5fb6159a2936a397f3b154170d555e
https://doi.org/10.1039/c5ra16095b
https://doi.org/10.1039/c5ra16095b
Autor:
Orla Sheils, F. von Stetten, Roland Zengerle, P. Saarinen, John J. O'Leary, Oliver Strohmeier, Thorsten Messinger, Simon Wadle, Gregor Czilwik, Daniel Mark, Günter Roth, J. Niittymäki, Nils Paust, K. McAllister
Publikováno v:
Lab on a Chip. 15:3749-3759
Diagnosis of infectious diseases suffers from long turnaround times for gold standard culture-based identification of bacterial pathogens, therefore impeding timely specific antimicrobial treatment based on laboratory evidence. Rapid molecular diagno
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa27db84956692f17975b427e4c5aafb
https://doi.org/10.1039/c5lc00591d
https://doi.org/10.1039/c5lc00591d