Novel Human Embryonic Stem Cell Regulators Identified by Conserved and Distinct CpG Island Methylation State.

Autor: Steve Pells, Eirini Koutsouraki, Sofia Morfopoulou, Sara Valencia-Cadavid, Simon R Tomlinson, Ravi Kalathur, Matthias E Futschik, Paul A De Sousa

Publikováno v: PLoS ONE, Vol 10, Iss 7, p e0131102 (2015)

Human embryonic stem cells (hESCs) undergo epigenetic changes in vitro which may compromise function, so an epigenetic pluripotency "signature" would be invaluable for line validation. We assessed Cytosine-phosphate-Guanine Island (CGI) methylation i

Externí odkaz: https://doaj.org/article/a23f5da6431c4b93b37c2f45b9627544

Thymic epithelial cell fate and potency in early organogenesis assessed by single cell transcriptional and functional analysis

Autor: Alison Mary Farley, An Chengrui, Sam Palmer, Dong Liu, Anastasia I. Kousa, Paul Rouse, Viktoria Major, Joanna Sweetman, Jan Morys, Andrea Corsinotti, Jennifer Nichols, Janice Ure, Renee McLay, Luke Boulter, S. Jon Chapman, Simon R. Tomlinson, C. Clare Blackburn

Publikováno v: Frontiers in Immunology, Vol 14 (2023)

During development, cortical (c) and medullary (m) thymic epithelial cells (TEC) arise from the third pharyngeal pouch endoderm. Current models suggest that within the thymic primordium most TEC exist in a bipotent/common thymic epithelial progenitor

Externí odkaz: https://doaj.org/article/9f035f9fc89d4b2ebc3e9d35b9da20b5

B1 SINE-binding ZFP266 impedes reprogramming through suppression of chromatin opening mediated by pioneering factors

Autor: Daniel F Kaemena, Masahito Yoshihara, James Ashmore, Meryam Beniazza, Suling Zhao, Mårten Bertenstam, Victor Olariu, Shintaro Katayama, Keisuke Okita, Simon R Tomlinson, Kosuke Yusa, Keisuke Kaji

Induced pluripotent stem cell reprogramming is inherently inefficient and understanding the molecular mechanisms underlying this inefficiency holds the key to successfully control cellular identity. Here, we report 16 novel reprogramming roadblock ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3d8d615fec0e012c23e431c35cd638a9
https://doi.org/10.1101/2022.01.04.474927

Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants

Autor: Simon R. Tomlinson, Kirsty M. Ferguson, Charles A. C. Williams, Richard A. Anderson, Neza Alfazema, Leanne Bradley, Benjamin Southgate, Alex von Kriegsheim, Maria Angeles Marques-Torrejon, Faye L Robertson, Gillian Morrison, Vivien Grant, Steven M. Pollard, Carla Blin, Jimi Wills, James Ashmore, Raul Bardini Bressan

Publikováno v: Bressan, R B, Southgate, B, Ferguson, K M, Blin, C, Grant, V, Alfazema, N, Wills, J C, Marques, M A, Morrison, G M, Ashmore, J, Robertson, F, Williams, C A C, Bradley, L, von Kriegsheim, A, Anderson, R A, Tomlinson, S R & Pollard, S M 2021, ' Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants ', Cell Stem Cell, vol. 28, no. 5, pp. 877-893.e9 . https://doi.org/10.1016/j.stem.2021.01.016

Point mutations within the histone H3.3 are frequent in aggressive childhood brain tumors known as pediatric high-grade gliomas (pHGGs). Intriguingly, distinct mutations arise in discrete anatomical regions: H3.3-G34R within the forebrain and H3.3-K2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b62ac005e0806ba597ff012910e68b7
https://www.pure.ed.ac.uk/ws/files/253710541/mmc4.pdf

Canonical notch signaling controls the early thymic epithelial progenitor cell state and emergence of the medullary epithelial lineage in fetal thymus development

Autor: Alison Farley, Freddy Radtke, Dong Liu, C. Clare Blackburn, Philip A. Seymour, Svetlana Ulyanchenko, Anastasia I. Kousa, Palle Serup, Martyna Popis, François Guillemot, Simon R. Tomlinson, Mette C. Jørgensen, Paul Rouse, Kathy E. O'Neill, Ute Koch

Publikováno v: Liu, D, Kousa, A, O'Neill, K, Rouse, P, Popis, M, Farley, A, Tomlinson, S, Ulyanchenko, S, Guillemot, F, Seymour, P A, Jorgensen, M, Serup, P, Koch, U, Radtke, F & Blackburn, C 2020, ' Canonical notch signaling controls the early thymic epithelial progenitor cell state and emergence of the medullary epithelial lineage in fetal thymus development ', Development . https://doi.org/10.1242/dev.178582
Development (Cambridge, England)
article-version (VoR) Version of Record
Liu, D, Kousa, A I, O'Neill, K E, Rouse, P, Popis, M, Farley, A M, Tomlinson, S R, Ulyanchenko, S, Guillemot, F, Seymour, P A, Jørgensen, M C, Serup, P, Koch, U, Radtke, F & Blackburn, C C 2020, ' Canonical Notch signaling controls the early thymic epithelial progenitor cell state and emergence of the medullary epithelial lineage in fetal thymus development ', Development (Cambridge, England), vol. 147, dev178582, pp. 1-16 . https://doi.org/10.1242/dev.178582

Thymus function depends on the epithelial compartment of the thymic stroma. Cortical thymic epithelial cells (cTECs) regulate T cell lineage commitment and positive selection, while medullary (m) TECs impose central tolerance on the T cell repertoire

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f59d4dfb94740ee3b061dd4b902fd68
https://www.pure.ed.ac.uk/ws/files/153893900/dev178582.full.pdf