Zobrazeno 1 - 10
of 107
pro vyhledávání: '"Simon J. Pilkis"'
Summary The aim of this work was to investigate the role of fructose 2,6-bisphosphate (Fru 2,6-P2) during photosynthesis. The level of Fru 2,6-P2 in tobacco plants was elevated by the introduction of a modified mammalian gene encoding 6-phosphofructo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4dbf2fd22c85a95fb299acc929d2ab71
https://ora.ox.ac.uk/objects/uuid:0734cb34-a024-4c57-ba5d-9b0e29a1dd98
https://ora.ox.ac.uk/objects/uuid:0734cb34-a024-4c57-ba5d-9b0e29a1dd98
Autor:
I. Deschamps, Graeme I. Bell, Philippe Passa, V. C. Pardini, Simon J. Pilkis, Irene T. Weber, Philippe Froguel, Gilberto Velho, C. Bellanné-Chantelot, Hélène Blanché, Jean-Jacques Robert, Martine Vaxillaire, Gregory M. Lipkind, D. Marotta, José Timsit
Publikováno v:
Diabetologia. 40:217-224
Mutations in glucokinase are associated with defects in insulin secretion and hepatic glycogen synthesis resulting in mild chronic hyperglycaemia, impaired glucose tolerance or diabetes mellitus. We screened members of 35 families with features of ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::153b75a60cabd3bb7bbceb40b8c95e33
https://doi.org/10.1007/s001250050666
https://doi.org/10.1007/s001250050666
Publikováno v:
Annual Review of Physiology. 58:171-186
The glycolytic enzyme glucokinase plays a key role in glucose sensing by the insulin-secreting pancreatic beta-cells, and mutations in the gene encoding this enzyme are a common cause of maturity-onset diabetes of the young (MODY), a form of non-insu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38f111654a72e6e4837a211fd4c8c31d
https://doi.org/10.1146/annurev.ph.58.030196.001131
https://doi.org/10.1146/annurev.ph.58.030196.001131
Autor:
M. Raafat El-Maghrabi, David A. Okar, Doriane Argaud, Alex J. Lange, Thomas C. Becker, Christopher B. Newgard, Simon J. Pilkis
Publikováno v:
Journal of Biological Chemistry. 270:24229-24236
6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase has been postulated to be a metabolic signaling enzyme, which acts as a switch between glycolysis and gluconeogenesis in mammalian liver by regulating the level of fructose 2,6-bisphosphate. The ef
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::495941642c9692cffa2b0ab9bd4c8eaa
https://doi.org/10.1074/jbc.270.41.24229
https://doi.org/10.1074/jbc.270.41.24229
Publikováno v:
Biochemical and Biophysical Research Communications. 213:663-672
Arg-104 of the kinase domain of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase was mutated to alanine, the mutant enzyme expressed in E. coli with a T7 RNA polymerase-based expression system, and purified to homogeneity by Blue-Sepharose and Q-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75a9fb04a0d474283f12e55bff31028d
https://doi.org/10.1006/bbrc.1995.2183
https://doi.org/10.1006/bbrc.1995.2183
Autor:
Simon J. Pilkis, Irwin J. Kurland
Publikováno v:
Protein Science. 4:1023-1037
The hepatic bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2-K/Fru-2,6-P2ase), E.C. 2.7-1-105/E.C. 3-1-3-46, is one member of a family of unique bifunctional proteins that catalyze the synthesis and degradation of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d14e5b5b5f9b4ed908b6bb33754c1b9d
https://doi.org/10.1002/pro.5560040601
https://doi.org/10.1002/pro.5560040601
Publikováno v:
Annual Review of Biochemistry. 64:799-835
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6211650e0c6217343ac3e99f2f7bd7ba
https://doi.org/10.1146/annurev.bi.64.070195.004055
https://doi.org/10.1146/annurev.bi.64.070195.004055
Publikováno v:
Journal of Biological Chemistry. 270:9939-9946
Glucokinase is distinguished from yeast hexokinase and low Km mammalian hexokinases by its low affinity for glucose and its cooperative behavior, even though glucose binding residues and catalytic residues are highly conserved in all of these forms o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7356408572e640105487baa42f11fdfc
https://doi.org/10.1074/jbc.270.17.9939
https://doi.org/10.1074/jbc.270.17.9939
Expression of Chicken Liver 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase in Escherichia coli
Publikováno v:
Biochemical and Biophysical Research Communications. 209:883-893
Chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was expressed in E. coli by using a pET3a T7 RNA polymerase-based expression system and was purified to homogeneity. The kinase and bisphosphatase of the expressed bifunctional enzyme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ab24478613ce0b4bacec5411b8a5a7a
https://doi.org/10.1006/bbrc.1995.1581
https://doi.org/10.1006/bbrc.1995.1581
Publikováno v:
Journal of Biological Chemistry. 269:27458-27465
The determinants of sugar specificity and cooperative behavior of human beta-cell glucokinase were studied by mutating several active site residues and performing a steady-state kinetic analysis of the purified mutant and wild-type enzymes after thei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::887f305fc6391a2d327c61cdf0ccd766
https://doi.org/10.1016/s0021-9258(18)47007-x
https://doi.org/10.1016/s0021-9258(18)47007-x