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Whereas the expression of foreign genes in mammalian cells usually proves successful, the purification of gene products is often a difficult and time-consuming process. The availability of monoclonal antibodies to the foreign protein can greatly assi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ce5b70863f214099a03c0567a8812dec
https://doi.org/10.1385/0-89603-204-3:461
https://doi.org/10.1385/0-89603-204-3:461
Publikováno v:
Immunochemical Protocols ISBN: 9780896034938
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4abdb64d9898eb4f96aca9ebd0afe21c
https://doi.org/10.1385/0-89603-493-3:427
https://doi.org/10.1385/0-89603-493-3:427
Publikováno v:
Molecular biotechnology. 1(3)
An expression system is described whereby a gene product is expressed fused to an antibody Fab fragment to form an antibody-like molecule. The antigen binding function of the original antibody is retained and the foreign gene replaces the CH2 and CH3
Publikováno v:
Biochemical Society transactions. 18(6)
Autor:
Robert B. Freedman, Simon J. Forster
Publikováno v:
Bioscience reports. 4(3)
Type-I procollagen, 14C-biosynthetically labelled, was reduced under denaturing and non-denaturing conditions. Reoxidation to disulphide-linked trimers occurred with non-denatured chains in the presence of an oxidant system containing oxidized and re
Publikováno v:
Biochemical Society Transactions. 14:756-757