Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Simon J. Bol"'
Publikováno v:
Scandinavian Journal of Haematology. 28:39-44
Separation of bone marrow cells in continuous bovine serum albumin density gradients and the agar culture technique were used to determine the buoyant density distribution of granulocyte-macrophage colony forming cells (CFUc) from normal marrow. The
Publikováno v:
British Journal of Haematology. 48:147-153
In tissue culture high proliferation potential colony forming cells (HPP-CFC, Bradly & Hodgson, 1979) formed large colonies in which macrophages predominated. Granulocyte/macrophage colony forming cells (GM-CFC, Bradley & Metcalf, 1966) formed smalle
Publikováno v:
Cytometry. 41:308-315
Background Megakaryocytes are classically identified by their cellular morphology and expression of platelet glycoproteins. Methods In this study, the expression of GPIIIa (CD61) on hemopoietic cells was analyzed by dual-fluorescence flow cytometry.
Publikováno v:
Leukemia Research. 19:113-120
The growth of B-cell precursor acute lymphoblastic leukemic (BCP ALL) cells in vitro is dependent on interactions with bone marrow (BM) stromal cells. We have recently demonstrated that the rate of cell division of BCP ALL cells increases when cultur
Publikováno v:
Leukemia Research. 18:337-346
Recent studies have confirmed that in vitro viability and proliferation of precursor B-cell leukaemia (ALL) cells are linked to the presence of bone marrow derived stromal cells. To investigate whether this effect is mediated by direct contact or thr
Publikováno v:
Leukemia Research. 18:37-48
A new assay system using the fluorescent probe PKH 26 GL was employed to investigate the regulation of precursor B-cell acute lymphoblastic leukaemic (ALL) cell growth. PKH 26 GL is a lipophilic fluorescent probe which becomes incorporated into the p
Publikováno v:
Leukemia Research. 17:873-882
The application of the fluorescent cell membrane probes PKH2 and PKH 26 GL in the measurement of leukaemic cell growth was examined on four cell lines K562, NALM-6, ACV (a pre-B cell line) and HL-60 using flow cytometry. As the amount of probe per ce
Publikováno v:
Leukemia research. 15(7)
In studies of in vitro leukemic clonogenic cells it is of importance to determine the cell lineage of individual clusters grown in culture. A method is described for the in-situ identification of leukemic cell clusters in methylcellulose cultures. Wh
Publikováno v:
Leukemia Research. 6:791-800
Bone marrow culture techniques and equilibrium density centrifugation of human bone marrow cells were used to analyse the neutrophil-granulocyte, macrophage and eosinophilgranulocyte progenitor hierarchy. The buoyant density of progenitor cells chang
Autor:
Neil Williams, Simon J. Bol
Publikováno v:
Journal of Cellular Physiology. 102:233-243
The progenitor cells of neutrophil granulocytes and macrophages which are able to proliferate and differentiate in vitro (CFU-c) form a heterogeneous population. By the use of specific colony stimulating activities and cell separation by equilibrium