In Search of Complementary Extraction Methods for Comprehensive Coverage of the Escherichia coli Metabolome

Autor: Henry Gould, William Cheung, James D. Finnigan, José Muñoz-Muñoz, Simon J. Charnock, Gary W. Black

Publikováno v: Metabolites, Vol 13, Iss 9, p 1010 (2023)

Escherichia coli is an invaluable research tool for many fields of biology, in particular for the production of recombinant enzymes. However, the activity of many such recombinant enzymes cannot be determined using standard biochemical assays, as oft

Externí odkaz: https://doaj.org/article/9100f8bd24d64e808d5ab900fc20a501

Regioselective Radical Alkylation of Arenes Using Evolved Photoenzymes

Autor: Claire G. Page, Jingzhe Cao, Daniel G. Oblinsky, Samantha N. MacMillan, Shiva Dahagam, Ruth M. Lloyd, Simon J. Charnock, Gregory D. Scholes, Todd K. Hyster

Publikováno v: Journal of the American Chemical Society. 145:11866-11874

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bd5616d4b4161814f436238974e62281
https://doi.org/10.1021/jacs.3c03607

Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes

Autor: Nicholas J. Weise, Syed T. Ahmed, Fabio Parmeggiani, James L. Galman, Mark S. Dunstan, Simon J. Charnock, David Leys, Nicholas J. Turner

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)

Abstract The suite of biological catalysts found in Nature has the potential to contribute immensely to scientific advancements, ranging from industrial biotechnology to innovations in bioenergy and medical intervention. The endeavour to obtain a cat

Externí odkaz: https://doaj.org/article/7bb9d4a74a89407ab9d72a5cb2e2d579

Enantioselective Synthesis of Pharmaceutically Relevant Bulky Arylbutylamines Using Engineered Transaminases

Autor: Yuriy V. Sheludko, Sjoerd Slagman, Samantha Gittings, Simon J. Charnock, Henrik Land, Per Berglund, Wolf‐Dieter Fessner

Publikováno v: Advanced Synthesis & Catalysis. 364:2972-2981

ATAs engineered for having an enlarged small binding pocket were applied for the synthesis of enantiomerically pure (R)-benzo[1,3]dioxol-5-yl-butylamine, a chiral component of human leukocyte elastase inhibitor DMP 777 (L-694,458). Kinetic resolution

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4c3723690242a52cfb32c9ca542f1a2
https://doi.org/10.1002/adsc.202200403

Synthesis of Pharmaceutically Relevant 2‐Aminotetralin and 3‐Aminochroman Derivatives via Enzymatic Reductive Amination

Autor: James R. Marshall, Han Bevinakatti, Joan Citoler, Nicholas J. Turner, James Finnigan, Vanessa Harawa, Simon J. Charnock

Publikováno v: Angewandte Chemie International Edition
Citoler, J, Harawa, V, Marshall, J R, Bevinakatti, H, Finnigan, J D, Charnock, S J & Turner, N J 2021, ' Synthesis of Pharmaceutically Relevant 2-Aminotetralin and 3-Aminochroman Derivatives via Enzymatic Reductive Amination ', Angewandte Chemie-International Edition, vol. 60, no. 46, pp. 24456-24460 . https://doi.org/10.1002/anie.202110321

2-Aminotetralin and 3-aminochroman derivatives are key structural motifs present in a wide range of pharmaceutically important molecules. Herein, we report an effective biocatalytic approach towards these molecules through the enantioselective reduct

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa1266e1ab5dbc46610326967a123e74
https://doi.org/10.1002/ange.202110321