Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Simon Boje Hansen"'
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2446
We have developed a generally applicable methodology for cysteine mutagenesis of nanobody (Nb) framework region serine residues. This strategy allows for subsequent labeling with thiol-reactive compounds without disrupting Nb antigen binding. We prov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ef02b20e6119c05669fb494075a19a3d
https://pubmed.ncbi.nlm.nih.gov/35157281
https://pubmed.ncbi.nlm.nih.gov/35157281
Publikováno v:
Methods in Molecular Biology ISBN: 9781071620748
Hansen, S B & Andersen, K R 2022, Introducing Cysteines into Nanobodies for Site-Specific Labeling . in G Hussack & K A Henry (eds), Single-Domain Antibodies : Methods and Protocols . Humana Press, New York, Methods in Molecular Biology, vol. 2446, pp. 327-343 . https://doi.org/10.1007/978-1-0716-2075-5_16
Hansen, S B & Andersen, K R 2022, Introducing Cysteines into Nanobodies for Site-Specific Labeling . in G Hussack & K A Henry (eds), Single-Domain Antibodies : Methods and Protocols . Humana Press, New York, Methods in Molecular Biology, vol. 2446, pp. 327-343 . https://doi.org/10.1007/978-1-0716-2075-5_16
We have developed a generally applicable methodology for cysteine mutagenesis of nanobody (Nb) framework region serine residues. This strategy allows for subsequent labeling with thiol-reactive compounds without disrupting Nb antigen binding. We prov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea6391d7a8636428125247ce0892f6f5
https://doi.org/10.1007/978-1-0716-2075-5_16
https://doi.org/10.1007/978-1-0716-2075-5_16
Autor:
Damiano Lironi, Simon Boje Hansen, Maria Vinther, Feng Feng, Giles E. D. Oldroyd, Zoltan Bozsoki, Christian Kofoed, Sébastien Fort, John T. Sullivan, Manoj Kamble, Ebbe Engholm, Knud J. Jensen, Noor de Jong, Kira Gysel, Clive W. Ronson, Christina Krönauer, Mikkel B. Thygesen, Mickaël Blaise, Simona Radutoiu, Kasper R. Andersen, Jens Stougaard
Publikováno v:
Science
Science, American Association for the Advancement of Science, 2020, 369 (6504), pp.663-670. ⟨10.1126/science.abb3377⟩
Bozsoki, Z, Gysel, K, Hansen, S B, Lironi, D, Krönauer, C, Feng, F, de Jong, N, Vinther, M, Kamble, M, Thygesen, M B, Engholm, E, Kofoed, C, Fort, S, Sullivan, J T, Ronson, C W, Jensen, K J, Blaise, M, Oldroyd, G, Stougaard, J, Andersen, K R & Radutoiu, S 2020, ' Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity ', Science (New York, N.Y.), vol. 369, no. 6504, pp. 663-670 . https://doi.org/10.1126/science.abb3377
Bozsoki, Z, Gysel, K, Hansen, S B, Lironi, D, Krönauer, C, Feng, F, De Jong, N, Vinther, M, Kamble, M, Thygesen, M B, Engholm, E, Kofoed, C, Fort, S, Sullivan, J T, Ronson, C W, Jensen, K J, Blaise, M, Oldroyd, G, Stougaard, J, Andersen, K R & Radutoiu, S 2020, ' Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity ', Science, vol. 369, no. 6504, pp. 663-670 . https://doi.org/10.1126/science.abb3377
Science, American Association for the Advancement of Science, 2020, 369 (6504), pp.663-670. ⟨10.1126/science.abb3377⟩
Bozsoki, Z, Gysel, K, Hansen, S B, Lironi, D, Krönauer, C, Feng, F, de Jong, N, Vinther, M, Kamble, M, Thygesen, M B, Engholm, E, Kofoed, C, Fort, S, Sullivan, J T, Ronson, C W, Jensen, K J, Blaise, M, Oldroyd, G, Stougaard, J, Andersen, K R & Radutoiu, S 2020, ' Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity ', Science (New York, N.Y.), vol. 369, no. 6504, pp. 663-670 . https://doi.org/10.1126/science.abb3377
Bozsoki, Z, Gysel, K, Hansen, S B, Lironi, D, Krönauer, C, Feng, F, De Jong, N, Vinther, M, Kamble, M, Thygesen, M B, Engholm, E, Kofoed, C, Fort, S, Sullivan, J T, Ronson, C W, Jensen, K J, Blaise, M, Oldroyd, G, Stougaard, J, Andersen, K R & Radutoiu, S 2020, ' Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity ', Science, vol. 369, no. 6504, pp. 663-670 . https://doi.org/10.1126/science.abb3377
Switching perception of friend and foe Lysine motif receptors in plants perceive glycans that signal the presence of pathogenic or symbiotic nitrogen-fixing microbes. Bozsoki et al. now define the portions of these receptors that create the discrimin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d91d42ec086089ec66bf2e10b93c314
https://hal.archives-ouvertes.fr/hal-02917628/document
https://hal.archives-ouvertes.fr/hal-02917628/document
Publikováno v:
'Acta Crystallographica D ', vol: 73, pages: 804-813 (2017)
Hansen, S B, Laursen, N S, Andersen, G R & Andersen, K R 2017, ' Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures ', Acta crystallographica Section D: Structural biology, vol. 73, no. Pt 10, pp. 804-813 . https://doi.org/10.1107/S2059798317013171
Acta Crystallographica. Section D, Structural Biology
Acta crystallographica / D 73(10), 804-813 (2017). doi:10.1107/S2059798317013171
Hansen, S B, Laursen, N S, Andersen, G R & Andersen, K R 2017, ' Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures ', Acta crystallographica Section D: Structural biology, vol. 73, no. Pt 10, pp. 804-813 . https://doi.org/10.1107/S2059798317013171
Acta Crystallographica. Section D, Structural Biology
Acta crystallographica / D 73(10), 804-813 (2017). doi:10.1107/S2059798317013171
Acta crystallographica / D 73(10), 804 - 813 (2017). doi:10.1107/S2059798317013171
The generation of high-quality protein crystals and the loss of phase information during an X-ray crystallography diffraction experiment represent the major bottl
The generation of high-quality protein crystals and the loss of phase information during an X-ray crystallography diffraction experiment represent the major bottl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acdf741b6d2018a28bf726caae8ee189
https://doi.org/10.1107/s2059798317013171
https://doi.org/10.1107/s2059798317013171