Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Simon A. Pfeiffer"'
Autor:
Ronny Frank, Heinz-Georg Jahnke, Andrea A. Robitzki, Laura Mauritz, Franziska D. Zitzmann, Felix Nitschke, Detlev Belder, Bernd Abel, Simon A. Pfeiffer
Publikováno v:
Analytical Chemistry. 89:13550-13558
For miniaturization and integration of chemical synthesis and analytics on small length scales, the development of complex lab-on-chip (LOC) systems is in the focus of many current research projects. While application specific synthesis and analytic
Autor:
Stefan Nagl, Christoph Schneider, Petra Glaeser, Detlev Belder, Simon A. Pfeiffer, Felix Nitschke, Matthias Spanka, Benjamin M. Rudisch, Andrea A. Robitzki
Publikováno v:
Analytical and Bioanalytical Chemistry. 410:853-862
Microreactors have gained increasing attention in their application toward continuous micro flow synthesis. An unsolved problem of continuous flow synthesis is the lack of techniques for continuous product purification. Herein, we present a micro fre
Publikováno v:
Microchimica Acta. 184:621-626
We describe a maskless photopolymerization technique for integration of luminescent sensors for determination of pH values and dissolved oxygen (DO) into commercially available all-glass microfluidic reactors (in sizes as small as 43 ± 3 μm for bot
Autor:
Andrea A. Robitzki, Elisabeth Speckmeier, Detlev Belder, Simon A. Pfeiffer, Kirsten Zeitler, Benjamin M. Rudisch, David Geissler
Publikováno v:
Analytical chemistry. 91(10)
The continuous separation mechanism of micro free-flow electrophoresis (μFFE) is a straightforward, suitable tool for microscale purification of reaction mixtures. However, aqueous separation buffers and organic reaction solvents limit the applicabi
Autor:
Erik Beckert, Detlev Belder, Christian Hoera, Mirjam M. Skadell, Maik Pahl, Simon A. Pfeiffer, Zhe Shu
Publikováno v:
Sensors and Actuators B: Chemical. 225:42-49
In this work, we present an approach for seamless integration of a highly variable flow rate sensor in chip based microfluidic devices. This novel, optically readable microfluidic calorimetric flow rate sensor is realized by a combination of an inkje
Autor:
Michael Gaebler, Marc Herm, Elisabeth Poehler, Benedikt Busse, Simon A. Pfeiffer, Stefan Nagl
Publikováno v:
Analytical and Bioanalytical Chemistry. 408:2927-2935
A pH probe with a microsecond luminescence lifetime was obtained via covalent coupling of 6-carboxynaphthofluorescein (CNF) moieties to ruthenium-tris-(1,10-phenanthroline)(2+). The probe was covalently attached to amino-modified poly-(2-hydroxyethyl
Publikováno v:
Engineering in Life Sciences. 15:276-285
A lifetime-based ratiometric microscale pH sensor system and a fluorescence microscopic setup was developed for the in-line observation of pH in free-flow isoelectric focusing based on the principle of time-domain dual lifetime referencing (t-DLR). T
Autor:
Carsten Lotter, Daniel Aigner, Christin Herzog, Elisabeth Poehler, Torsten Mayr, Simon A. Pfeiffer, Stefan Nagl
Publikováno v:
The Analyst. 140:7496-7502
We demonstrate the fabrication, characterization and application of microfluidic chips capable of continuous electrophoretic separation via free flow isoelectric focussing (FFIEF). By integration of a near-infrared (NIR) fluorescent pH sensor layer u
Application of Optical pH Sensors in the Microfluidic Free-flow Isoelectric Focusing of Biomolecules
Autor:
Daniel Aigner, Stefan Nagl, Erik Beckert, Andrea J. Peretzki, Carsten Lotter, Simon A. Pfeiffer, Elisabeth Poehler, Torsten Mayr, Christin Herzog
Publikováno v:
Procedia Engineering. 120:175-179
We present methods for fabrication and application of microfluidic chips for free-flow electrophoresis (FFE) that allow integrated pH sensing. Monitoring of the pH gradient in microfluidic free-flow isoelectric focusing (FFIEF) allows on-line adjustm
Autor:
Simon A. Pfeiffer, Stefan Nagl
Publikováno v:
Analytical chemistry. 89(17)
Optical absorbance detection based on attenuated light transmission is limited in sensitivity due to short path lengths in microfluidic and other miniaturized platforms. An alternative is detection using the photothermal effect. Herein we introduce a