Staphylococcal Protein A Is a Key Factor in Neutrophil Extracellular Traps Formation

Autor: Tamara Hoppenbrouwers, Andi R. Sultan, Tsion E. Abraham, Nicole A. Lemmens-den Toom, Silvie Hansenová Maňásková, Wiggert A. van Cappellen, Adriaan B. Houtsmuller, Willem J. B. van Wamel, Moniek P. M. de Maat, Johan W. van Neck

Publikováno v: Frontiers in Immunology, Vol 9 (2018)

Staphylococcus aureus are strong inducers of neutrophil extracellular traps (NETs), a defense mechanism of neutrophils against pathogens. Our aim was to explore the role of Protein A in S. aureus-induced NETosis. We determined the Protein A productio

Externí odkaz: https://doaj.org/article/f4475e7b910646f4839b0bfbaae80543

Synthetic LPETG-containing peptide incorporation in the Staphylococcus aureus cell-wall in a sortase A- and growth phase-dependent manner.

Autor: Silvie Hansenová Maňásková, Kamran Nazmi, Alex van Belkum, Floris J Bikker, Willem J B van Wamel, Enno C I Veerman

Publikováno v: PLoS ONE, Vol 9, Iss 2, p e89260 (2014)

The majority of Staphylococcus aureus virulence- and colonization-associated surface proteins contain a pentapeptide recognition motif (LPXTG). This motif can be recognized and cleaved by sortase A (SrtA) which is a membrane-bound transpeptidase. Aft

Externí odkaz: https://doaj.org/article/1ea36ac7e5f44fbc91232c60262345d5

Incorporation of a Valine–Leucine–Lysine-Containing Substrate in the Bacterial Cell Wall

Autor: Wendy E. Kaman, Floris J. Bikker, Johan A. Slotman, Silvie Hansenová Maňásková, Wiggert A. van Cappellen, Kamran Nazmi, Enno C. I. Veerman, Adriaan B. Houtsmuller, Rianne van Zuidam

Publikováno v: Bioconjugate chemistry, 27(10), 2418-2423. American Chemical Society
Hansenova Manaskova, S, Bikker, F J, Nazmi, K, van Zuidam, R, Slotman, J A, van Capellen, W A, Houtsmuller, A B, Veerman, E C I & Kaman, W E 2016, ' Incorporation of a valine−leucine−lysine-containing substrate in the bacterial cell wall ', Bioconjugate chemistry, vol. 27, no. 10, pp. 2418-2423 . https://doi.org/10.1021/acs.bioconjchem.6b00381
Bioconjugate Chemistry, 27(10), 2418-2423. American Chemical Society

The emergence of antibiotic-resistant bacteria is a major public health threat, and therefore novel antimicrobial targets and strategies are urgently needed. In this regard, cell-wall-associated proteases are envisaged as interesting antimicrobial ta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f303af26f46f19e783040d1fbbd60114
https://doi.org/10.1021/acs.bioconjchem.6b00381

Production of Staphylococcal Complement Inhibitor (SCIN) and Other Immune Modulators during the Early Stages of Staphylococcus aureus Biofilm Formation in a Mammalian Cell Culture Medium

Autor: Andi R. Sultan, Annelies Verbon, Susan V. Snijders, Jasper Swierstra, Silvie Hansenová Maňásková, Willem J. B. van Wamel, Nicole Lemmens-den Toom

Publikováno v: Infection & Immunity, 86(8):e00352-18. American Society for Microbiology

Immune modulators are known to be produced by matured biofilms and during different stages of planktonic growth of Staphylococcus aureus. Little is known about immune modulator production during the early stages of biofilm formation, thus raising the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c935d8e70926b44524549b7703066219
https://doi.org/10.1128/iai.00352-18

Staphylococcus aureus sortase a-mediated incorporation of peptides

Autor: Kamran Nazmi, Floris J. Bikker, Willem J. B. van Wamel, Wim van 't Hof, Alex van Belkum, Nathaniel I. Martin, Enno C. I. Veerman, Silvie Hansenová Maňásková

Publikováno v: PLoS ONE
PLoS One, 11(1). Public Library of Science
Hansenová Maňásková, S, Nazmi, K, van 't Hof, W, van Belkum, A, Martin, N I, Bikker, F J, van Wamel, W J B & Veerman, E C I 2016, ' Staphylococcus aureus sortase a-mediated incorporation of peptides : Effect of peptide modification on incorporation ', PLoS ONE, vol. 11, no. 1, e01474 . https://doi.org/10.1371/journal.pone.0147401
PLoS ONE, 11(1):e01474. Public Library of Science
PLoS ONE, 11(1), e147401
PLoS ONE, Vol 11, Iss 1, p e0147401 (2016)
PLoS One (print), 11(1). Public Library of Science

The endogenous Staphylococcus aureus sortase A (SrtA) transpeptidase covalently anchors cell wall-anchored (CWA) proteins equipped with a specific recognition motif (LPXTG) into the peptidoglycan layer of the staphylococcal cell wall. Previous in sit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47ed404e0e496f2163dae038ceb0b4ad
https://hdl.handle.net/11245/1.542936